天道酬勤[HPLC法分析川芎-香附配伍前后指标成分的变化]柴胡香附川芎作者简介]张文娟，女，主管药师，硕士，主要从事中药化学成分分析。[通讯作者]王庆伟，男，主管药师，博士，主要从事药物代谢动力学及透皮制剂的研究。[]目的：研究药对川芎-香附配伍对单味药材中指标成分的影响。方法：以川芎嗪、阿魏酸、藁本内酯和α-香附酮为指标成分，采用HPLC法分析川芎、香附及川芎-香附配伍前后变化。色谱条件：色谱柱为InertsilODS-3〔4.6mm×250mm，5μm〕；流动相为：甲醇-0.05%磷酸水溶液〔梯度洗脱，0~20min：45%~48%甲醇；20~25min：48%~70%甲醇；25~50min：70%~75%甲醇；50~55min：75%~100％甲醇；55~60min：100%~45%甲醇〕，检测波长为270nm。结果：药对中指标成分川芎嗪、阿魏酸、藁本内酯含量与单味药材相比均有所增大。结论：川芎-香附配伍前后特征峰面积均发生明显变化。[关键词]HPLC；川芎-香附；川芎嗪；阿魏酸；藁本内酯；α-香附酮[中图分类号]R284.2[文献标识码]A[文章编号]1673-7210〔2023〕07〔b〕-096-03AnalysisofthediversifyinthematchedpairofChuanxiongRhizomaandCyperiRhizomabyHPLCZHANGWenjuan,XUYuan,WANGQingweiDepartmentofPharmacy,TangduHospital,theFourthMilitaryMedicalUniversity,Xi′an710038,China[Abstract]Objective:TostudytheeffectontheindexcomponentofChuanxiongRhizomaandCyperiRhizomaaftermatched.Methods:Theligustrazine,ferulicaidandligustilidewereusedastheindexingredientstoanalyzethediversityinthematchedpairofChuanxiongRhizomaandCyperiRhizomabyHPLC.TheInertsilODS-3(4.6mm×250mm,5μm)columnwasused;methanol-0.05%phosphoricacidwasusedasmobilephaseinagradientelution(0-20min:45%-48%methanol;20-25min:48%-70%methanol;25-50min:70%-75%methanol;50-55min:75%-100%methanol;55-60min:100%-45%methanol).Thedetectivewavelengthwas270nm,andtheflowratewasabout1ml/min.Results:Theconcentrationofligustrazine,ferulicacidandligustilideincrudedrugpairswereincreasedcomparedwiththesingleplantmedicine.Conclusion:Thepeakareahaschangedabsolutelyaftermatched.[Keywords]HPLC;ThematchedpairofChuanxiongRhizomaandCyperiRhizoma;Ligustrazine;Ferulicacid;Ligustilide;α-Cyperone川芎-香附既是行气疏肝的药对，也是很多大复方中常用的配伍结构，临床主要用于郁证的治疗。...
