3062023,Vol.44,No.04食品科学※安全检测基于Cu,Fe-N-C氧化酶活性构建邻苯二甲酸酯荧光检测探针李宏1,2，史巧1，陈骏飞1，王馨蕊1，汤回花1，李秋兰2，杨德志2，杨亚玲2,*（1.云南省农业科学院农产品加工研究所，云南昆明650221；2.昆明理工大学生命科学与技术学院，云南昆明650500）摘要：基于邻苯二甲酸酯（phthalicacidester，PAEs）对双金属原子Cu,Fe-N-C纳米酶模拟氧化酶活性的调节，建立简单、灵敏测定发酵食品中PAEs的荧光探针方法。PAEs吸附于Cu,Fe-N-C表面后，会加速Cu,Fe-N-C催化氧化底物邻苯二胺，使生成的2,3-二氨基吩嗪产物荧光强度增加，PAEs在1.25～50μg/L范围内呈良好的线性关系。将该研究方法用于PAEs含量的测定，其检出限可达到0.76μg/L，实际样品的加标回收率在88.0%～104.6%范围内，满足GB5009.271—2016中的检测要求。该方法具有简便、稳定、快速等特点，其检出限也低于国家规定的食品中PAEs含量的限值。关键词：邻苯二甲酸酯；纳米酶；氧化酶活性；荧光探针AFluorescentProbeBasedonCu,Fe-N-CNanozymewithOxidase-likeActivityforDetectionofPhthalateEstersLIHong1,2,SHIQiao1,CHENJunfei1,WANGXinrui1,TANGHuihua1,LIQiulan2,YANGDezhi2,YANGYaling2,*(1.InstituteofAgro-productsProcessing,YunnanAcademyofAgriculturalSciences,Kunming650221,China;2.FacultyofLifeScienceandTechnology,KunmingUniversityofTechnology,Kunming650500,China)Abstract:Asimpleandsensitivefluorescentprobeforthedeterminationofphalicacidester(PAEs)infermentedfoodswasdevelopedbasedonthefactthattheoxidase-likeactivityofCu,Fe-N-CnanozymecanberegulatedbyPAEs.AfterPAEswereadsorbedonthesurfaceofCu,Fe-N-C,thecatalyticoxidationofthesubstrateo-phenylenediamine(OPD)wasaccelerated,thusincreasingthefluorescenceintensityofthegenerated2,3-diaminophenazine(DAP).ThecalibrationcurveforPAEsshowedagoodlinearrelationshipintheconcentrationrangeof1.25–50μg/L.Thedetectionlimitoftheproposedmethodwas0.76μg/L.Therecoveriesofspikedsamplesrangedfrom88.0%to104.6%,whichmeetthedetectionrequirementsofthenationalstandardGB5009.271-2016.Thismethodissimple,stableandrapid,anditsdetectionlimitislowerthanthenationallimitforPAEscontentinfoods.Keywords:phthalicacidesters;nanozyme;oxidase-likeactivi...
