肿瘤防治研究2023年第50卷第1期CancerResPrevTreat,2023,Vol.50,No.1·63·doi:10.3971/j.issn.1000-8578.2023.22.0483·临床研究·骨肉瘤中PD-1和CTLA-4的表达与患者临床病理特征及预后的相关性闫广宁，喻玲，赖续文，叶丹丽，王蔚，王卓才CorrelationBetweenPD-1/CTLA-4ExpressionswithClinicopathologicalFeaturesandPrognosisofOsteosarcomaPatientsYANGuangning,YULing,LAIXuwen,YEDanli,WANGWei,WANGZhuocaiDepartmentofPathology,GeneralHospitalofSouthernTheatreCommand,Guangzhou510010,ChinaCorrespondingAuthor:WANGZhuocai,E-mail:wangzcmail@163.comAbstract:ObjectiveToexploretheexpressionofPD-1andCTLA-4inosteosarcomaandtheirclinicalsignificance.MethodsFifty-eightcasesofosteosarcomaencounteredfrom2007to2016wereenrolled.TheexpressionlevelsofPD-1andCTLA-4weredetectedthroughimmunohistochemistry(EnVisionmethod).ResultsPD-1waspositivelyexpressedin31(53.4%)casesandnegativelyexpressedin27(46.6%)cases.CTLA-4waspositivelyexpressedin19(32.8%)casesandnegativelyexpressedin39(67.2%)cases.Atotalof12(20.7%)caseswerePD-1andCTLA-4doublepositive,whereas20(34.5%)casesweredoublenegative,and26(44.8%)casesweresinglepositive.ThepositiveexpressionofPD-1wascorrelatedwithneoadjuvantchemotherapy,tumormetastasisandrelapse,andshortenedsurvivaltime(P<0.05).ThepositiveexpressionofCTLA-4waspartlyrelatedwithlateEnneckingstage(P=0.051).Doublepositiveexpressionwasrelatedtothehighesttumormetastasisandrelapseratesandtheworstprognosis(P<0.05),comparedwithdoublenegativeandsinglepositiveexpression.ConclusionPositiveexpressionofPD-1andCTLA-4inosteosarcomaisassociatedwithworseprognosis,whereasdoublepositiveexpressionisassociatedwiththehighesttumorrelapseandmetastasisratesandshortestsurvivaltime.Theseresultsarepotentialvaluablereferencesforosteosarcomaimmunotherapy.Keywords:Osteosarcoma;PD-1;CTLA-4;PrognosisFunding:YouthProgramofNationalNaturalandScienceFoundationofChina(No.81802962)Competinginterests:Theauthorsdeclarethattheyhavenocompetinginterests.摘要：目的探讨PD-1和CTLA-4在骨肉瘤中的表达及其临床病理意义。方法收集2007—2016年南部战...
