农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2805-28092022年12月张寅寅，黑多尔，刘玥婷，等.荷兰进境百合种球中线虫的分离及分子鉴定[J].农业环境科学学报,2022,41（12）：2805-2809.ZHANGYY,HEIDE,LIUYT,etal.IsolationandmolecularidentificationofnematodesinterceptedfromimportedNetherlands′lilybulbs[J].JournalofAgro-EnvironmentScience,2022,41（12）：2805-2809.开放科学OSID荷兰进境百合种球中线虫的分离及分子鉴定张寅寅，黑多尔，刘玥婷，李鑫*（大连海关技术中心，辽宁大连116001）IsolationandmolecularidentificationofnematodesinterceptedfromimportedNetherlands′lilybulbsZHANGYinyin,HEIDuoer,LIUYueting,LIXin*（TechnologyCenterofDalianCustoms,Dalian116001,China）Abstract：TheincreasingimportoftheNetherlands′lilybulbsovertheyearshasmeantintroductionofavarietyofpestsintothecountry;andonesuchnematodepesthasturnedintorisk,endangeringthenationalbiosafety.Quarantineandidentificationofnematodesarethusimperative.Thispaperprovidesreferenceforthequarantineofimportedseedlingsandflowerstovariousportsandthesupervisionofplantingsites.Inthisstudy,morphology（Beemanfunnelmethod）,combinedwithmolecularbiologicalmethods（DNAextractionandPCRamplification）,wereusedtoquarantineandidentifyplantparasiticnematodesisolatedfromlilybulbs.Theresultsshowedthatthreespeciesofplantparasiticnematodeswerefound：Pratylenchuspenetrans,Aphelenchoidesblastophthorus,andAphelenchusavenae.Theresultsalsoindicatedthatthequantityofnematodesisolatedfromtheimportedlilybulbswaslimited,withmostbeinglarvae,asisitdifficulttoaccuratelyidentifynematodesbasedontheirmorphology,thisprocessneededtobesupplementedbymolecularbiologicalmethodstoensuretheaccuracyoftheresults.Keywords：Netherlands;lilybulb;nematode;molecularidentification收稿日期：2022-11-10录用日期：2022-12-06作者简介：张寅寅（1987—），女，辽宁大连人，硕士，从事植物检疫工作。E-mail：2035425610@qq.com黑多尔与张寅寅同等贡献*通信作者：李鑫E-mail：14826155@qq.com基金项目：“十四五”国家重点研发计划课题（2021YFD1400100）Projectsupported：TheNationalKeyResearc...
