作者简介:王亮,男,技师,主要从事药物浓度监测及相关代谢基因研究。·论著·DOI:10.3969/j.issn.1672-9455.2023.04.011高效液相色谱外标法检测人血清中拉莫三嗪、卡马西平及单羟奥卡西平的血药浓度王亮郑州大学附属洛阳中心医院医学检验科,河南洛阳471000摘要:目的建立同时检测人血清中卡马西平、拉莫三嗪及单羟奥卡西平浓度的高效液相色谱外标法。方法采用高效液相色谱外标法进行血药浓度测定,血清样品用甲醇去除蛋白,用C18色谱柱(4.6mm×250.0mm,5.0μm)分析,流动相为甲醇水溶液(其比例为60∶40),流速为1.0mL/min,柱温为35℃,检测波长为240nm。同时考察该方法的专属性、标准曲线及最低检测限、精密度与回收率、稳定性及重复性。结果卡马西平、拉莫三嗪、单羟奥卡西平浓度分别在0.25~4.00μg/mL(Y=15032.4X+22918.4,r=0.996),0.25~4.00μg/mL(Y=17249.7X+52954.8,r=0.992)及0.75~12.00μg/mL(Y=6268.65X+28631.3,r=0.995)线性关系良好。卡马西平、拉莫三嗪、单羟奥卡西平的方法回收率均为95%~106%,日内和日间精密度均<5%。结论该方法操作简便、准确、灵敏度高,可用于卡马西平、拉莫三嗪及单羟奥卡西平血药浓度监测。关键词:拉莫三嗪;卡马西平;单羟奥卡西平;高效液相色谱外标法;血药浓度中图法分类号:R917文献标志码:A文章编号:1672-9455(2023)04-0481-04Determinationofconcentrationsoflamotrigine,carbamazepineandmonohydroxyoxcarbazepineinhumanbloodbyexternalstandardHighPerformanceLiquidChromatographymethodofHPLCWANGLiangDepartmentofClinicalLaboratory,LuoyangCentralHospitalAffiliatedtoZhengzhouUniversity,Luoyang,Henan471000,ChinaAbstract:ObjectiveToestablishanexternalstandardHighPerformanceLiquidChromatographymethodtodeterminatetheconcentrationsoflamotrigine,carbamazepineandmonohydroxyoxcarbazepinesimultane-ouslyinhumanblood.MethodsTheproteinsintheserumwereremovedwithmethanol,theircontentsweredeterminedbyusingtheexternalstandardHighPerformanceLiquidChromatographymethod.ThemethodwasperformedontheC18column(4.6mm×250.0mm,5.0μm).Themobilephaseconsistedofmethanol-water(60∶40),theflowratewas1.0mL/minandthedetectingwavelengthwas240nm.Themethodwasin-spectedintheaspectsofspecificity,lowerlimitofquantification,precision,recoveryrate,stabilityandrepeat-ability.ResultsThe...
