《食品工业》2023年第44卷第2期300分析检测高效液相色谱法快速测定白酒中纽甜的含量许文雅，张宏宏，林洋，刘瀛，郁蕉竹，鞠晓莹沈阳市食品药品检验所（沈阳110122）摘要研究建立了高效液相色谱法快速测定白酒中纽甜的含量,简化了国标中对样品的前处理过程,优化了色谱条件。白酒除乙醇后用水定容上机,采用ThermoC18色谱柱,以乙腈-辛烷磺酸钠溶液=40∶60(V/V)为流动相,采用二极管阵列检测器,检测波长210nm,柱温30℃,流速1mL/min。结果表明,在该色谱条件下,纽甜在0.2~10mg/L的质量浓度范围内与峰面积具有良好的线性关系,相关系数R值为0.999978,方法检出限为0.06mg/kg,定量限为0.2mg/kg,3个不同添加量0.2,0.5和1.0mg/kg水平下纽甜加标平均回收率为97.1%~99.0%,相对标准偏差(n=6)为0.39%~1.3%。该方法前处理简单、快速,在该色谱条件下分离效果好,整个检验过程分析成本低,为检测白酒中的纽甜含量提供了更快捷的方法。关键词纽甜;白酒;高效液相色谱法;快速测定RapidDeterminationofNeotameinLiquorbyHighPerformanceLiquidChromatographyXUWenya,ZHANGHonghong,LINYang,LIUYing,YUJiaozhu,JUXiaoyingShenyangInstituteforFoodandDrugControl(Shenyang110122)AbstractAhighperformanceliquidchromatographymethodwasestablishedfortherapiddeterminationofneotameinliquor,whichsimplifiedthepretreatmentprocessofthesamplesinthenationalstandardandoptimizedthechromatographicconditions.Afterremovingtheethanol,theliquorwasdirectlyconnectedtothemachine,neotamewasqualitativelyanalyzedonaThermoC18columnwithadiodearraydetectorat210nmusingacetonitrile-sodiumoctanesulfonatesolutionof40∶60(V/V)asthemobilephase,thecolumntemperaturewas30℃,andtheflowratewas1mL/min.Theresultsshowedthatunderthechromatographicconditions,neotamehadagoodlinearrelationshipwithpeakareainthemassconcentrationrangeof0.2-10mg/L,thecorrelationcoefficientwas0.999978,thelimitofdetectionwas0.06mg/kg,andthelimitofquantificationwas0.2mg/kg.Theaveragerecoveriesofneotamespikedatthelevelsof0.2,0.5and1.0mg/kgwere97.1%-99.0%,andtherelativestandarddeviation(n=6)was0.39%-1.3%.Themethodwassimpleandrapidinpretreatment,whichhadgoodseparationeffectandlowanalysiscostinthewholeinspectionprocess,andprovidedamethodfortherapiddeter...
