枸杞多糖对睡眠剥夺大鼠血液...胞亚群及相关炎性因子的影响_栾和芝.pdf

职业与健康2023 年 2 月第 39 卷第 3 期Occup and Health，Feb.2023，Vol.39，No.3作者简介：栾和芝，女，主管技师，主要从事检验学的临床与研究工作。通信作者：单铁强，副主任技师，E-mail：枸杞多糖对睡眠剥夺大鼠血液中 T 细胞亚群及相关炎性因子的影响栾和芝1，单铁强2，姜柳2，韩长海2，祁皎2，吕晶31.河北港口集团有限公司港口医院检验科，河北 秦皇岛 066000；2.秦皇岛市海港医院检验科，河北 秦皇岛 066000；3.河北工程大学医学院，河北 邯郸 056002摘要：目的分析枸杞多糖（Lycium barbarum polysaccharides，LBP）对睡眠剥夺大鼠血液中 T 细胞亚群型的数量、相关炎性因子以及大鼠行为能力的影响，探讨 LBP 对睡眠剥夺大鼠的免疫保护机制。方法将 SD 大鼠通过“多平台水环境”技术成功制备剥夺睡眠大鼠模型，按照干预方法将其均等分成 3 组（模型组、LBP 干预组及西药干预组），每组 6 只，同时把 6 只健康同种属大鼠作为对照组。在建模成功后 4 组鼠每天的干预用药不同，LBP 干预组：灌服 LBP（0.54 mg/kg），西药干预组：灌服舒乐安定（6 g/kg），模型组和对照组：灌服等容量三蒸水，持续用药 4 周。检测血液中 T 淋巴细胞亚群 CD3+T 细胞、CD4+T细胞、CD8+T 细胞的比例以及 CD4+T/CD8+T 的比值；ELISA 检测血液中肿瘤坏死因子-（tumor necrosis factor-，TNF-）、-干扰素（interferon-，IFN-）、白介素-1（interleukin-1，IL-1）、白介素-2（interleukin-2，IL-2）及白介素-6（interleukin-6，IL-6）的含量；检测各组大鼠的行为学指标以衡量其大脑学习记忆功能的变化。结果和对照组相比，模型组大鼠血液中CD4+T 细胞比例减少（25.642.53）%，CD8+T 细胞比例增多（20.712.21）%，CD4+T/CD8+T 比值（1.660.13）降低，血液中的炎性因子：TNF-（15.992.02）ng/mL、IFN-（3.220.45）ng/mL、IL-1（22.702.87）pg/mL、IL-2（15.851.77）ng/mL及IL-6（64.656.23）pg/mL的含量均增加，大鼠的行为能力（42.364.60）s，（10.261.68）次升高（均 P0.01）；和模型组相比，LBP 干预组大鼠血液中 CD4+T 细胞比例升高（35.633.00）%、CD8+T 细胞比例降低（14.101.94）%，CD4+T/CD8+T 比值（2.380.21）增加，血液中的炎性因子：TNF-（10.251.72）ng/mL、IFN-（2.130.34）ng/mL、IL-1（16.261.80）pg/mL、IL-2（11.901.50）ng/mL及 IL-6（46.744.39）pg/mL的含量均下降，大鼠的行为能力（31.513.98）s，（16.902.72）次降低（均 P0.05）。结论LBP 能够通过调节大鼠血液中 T细胞亚型的数量及降低炎性因子的含量来提高睡眠剥夺大鼠的行为能力。关键词：枸杞多糖；睡眠剥夺；T 细胞亚群；炎性因子；行为能力中国图书资料分类号：R-332文献标识码：A文章编号：1004-1257（2023）03-0317-05Effects of Lycium barbarum polysaccharides on T cell subtypes and related inflammatoryfactors in blood of sleep deprived ratsLUAN He-zhi1，SHAN Tie-qiang2，JIANG Liu2，HAN Chang-hai2，QI Jiao2，LYU Jing31.Laboratory Department，Port Hospital of Hebei Port Group Co.，Ltd，Qinhuangdao Hebei，066000，China；2.Department ofLaboratory，Qinhuangdao Haigang Hospital，Qinhuangdao Hebei，066000，China；3.Medical College，Hebei Engineering University，Handan Hebei，056002，ChinaCorresponding author：SHAN Tie-qiang，Deputy chief technician，E-mail：Abstract：Objective To analyze the effects of Lycium barbarum polysaccharide（LBP）on the number of T cell subtypes，relatedinflammatory factors and behavioral ability of sleep deprived rats，explore the immune protective mechanism of LBP on sleepdeprived rats.Methods SD rats were successfully prepared into sleep deprivation rat models by multiplatform waterenvironment technology.According to the intervention methods，they were equally divided into three groups（model group，LBPintervention group and western medicine intervention group）with six rats in each group，at the same time，six healthy rats of thesame genus were used as the control group.After successful modeling，the intervention drugs of the four groups were differentevery day.LBP intervention group was given LBP（0.54 mg/kg）orally，western medicine intervention group was given estazolam（6g/kg）orally，and model group and control group were given equal volume three distilled water orally，continuous medication forfour weeks.The number of T lymphocyte subsets CD3+T cells，CD4+T cells，and the ratio of CD4+T/CD8+T in the blood were 论著 317DOI:10.13329/ki.zyyjk.2023.0067职业与健康2023 年 2 月第 39 卷第 3 期Occup and Health，Feb.2023，Vol.39，No.3detected.The contents of tumor necrosis factor-（TNF-），-interferon（IFN-），interleukin-1（IL-1），interleukin-2（IL-2）andinterleukin-6（IL-6）in blood.The behavioral indicators of rats in four groups were tested to measure the changes of brain learningand memory function.Results Compared with the control group，the percentage of CD4+T cells in the blood of model groupdecreased（25.642.53）%，the percentage of CD8+T cells increased（20.712.21）%，the ratio of CD4+T/CD8+T decreased（1.660.13），the contents of the inflammatory factor in the blood increased，which included TNF-（15.992.02）ng/mL，IFN-（3.220.45）ng/mL，IL-1（22.702.87）pg/mL，IL-2（15.851.77）ng/mL and IL-6（64.656.23）pg/mL，and the behavioral ability ofrats（42.364.60）s，（10.261.68）times increased（all P0.01）.Compared with the model group，the percentage of CD4+T cells inthe blood of LBP intervention group increased（35.633.00）%，the percentage of CD8+T cells decreased（14.101.94）%，theratio of CD4+T/CD8+T increased（2.380.21），the contents of the inflammatory factor in the blood decreased，which included TNF-（10.251.72）ng/mL，IFN-（2.130.34）ng/mL，IL-1（16.261.80）pg/mL，IL-2（11.901.50）ng/mL and IL-6（46.744.39）pg/mL，and the behavioral ability of rats（31.513.98）s，(16.902.72）times decreased（all P0.05）.Conclusion Lycium barbarumpolysaccharide can improve the behavioral ability of sleep deprived rats by regulating the number of T cell subtypes and reducingthe content of inflammatory factors in blood.Keywords：Lycium barbarum polysaccharide；Sleep deprivation；T cell subtypes；Inflammatory factors；Behavioral ability在当今快速发展的信息时代下，大多数人们的作息时间不规律，出现睡眠时间减少，甚至失眠现象。若人的睡眠时间长期达不到其所需的正常生理要求时，称这种现象为睡眠剥夺。此种患者会出现程度不等的头痛、乏力和关节痛等身体不适，同时会出现专注力下降、学习记忆力差，焦虑及抑郁等症状1-2，对患者的生活、工作及学习均造成程度不等的负面影响。至今，医学专家对于睡眠剥夺的发病机制尚无统一定论。近年来，有较多的研究着重探索睡眠剥夺对机体免疫因素的影响，发现睡眠剥夺能造成机体免疫器官功能低下及免疫细胞活性降低3-4。枸杞多糖（Lycium barbarumpolysaccharides，LBP）