宫颈癌组织中NEK2和ci...疗敏感度及预后的相关性研究_陈霄.pdf

27现代检验医学杂志第 38 卷第 1 期2023 年 1 月J Mod Lab Med,Vol.38,No.1,Jan.2023宫颈癌组织中 NEK2 和 circPITX1 的表达水平与 放疗敏感度及预后的相关性研究陈霄，吴小颖，安明（四川大学华西三亚医院妇产科，海南三亚 572000）摘要：目的探讨宫颈癌组织中中心体相关激酶 2(never in mitosis geneA related kinase-2，NEK2)和环状 RNA 配对同源结构域基因1(circRNA paired homology domain gene 1，circPITX1)表达与放疗敏感度及预后的关系。方法选取 2017 年1月2018 年1月四川大学华西三亚医院接受放疗治疗的 82 例宫颈癌患者为研究对象。依据放疗后病灶退缩情况评价放疗疗效,分为放疗抵抗组（n=51）和放疗敏感组（n=31）。应用免疫组织化学法（immunohistochemistry，IHCS）检测宫颈癌癌组织和癌旁组织中 NEK2 蛋白表达。采用荧光实时定量 PCR（real-time quantitative PCR，RT-qPCR）检测 circPITX1mRNA 和 NEK2 mRNA 表达。统计学分析癌组织中circPITX1mRNA 和 NEK2 mRNA 与放疗敏感度、临床病理特征的关系。采用 Kaplan-Meier 生存曲线分析宫颈癌患者 circPITX1mRNA 和 NEK2 mRNA 表达水平与患者生存预后的关系。COX 回归分析宫颈癌患者预后不良的危险因素。结果宫颈癌癌组织中 NEK2 蛋白表达阳性率 85.37%（70/82）高于癌旁组织 12.20%（10/82），差异有统计学意义（2=87.857，P 0.05）。癌组织中circPITX1mRNA（1.920.24），NEK2 mRNA 表达（2.040.31）高于癌旁组织（0.810.20，0.930.27）表达，差异均有统计学意义（t=32.174，24.450，均 P 0.05）。宫颈癌组织中 NEK2 mRNA与circPITX1mRNA 表达呈显著正相关(r=0.618,P 0.05)。FIGO 分期 A B 期宫颈癌患者 NEK2 mRNA（2.540.23），circPITX1mRNA（2.330.19）表达明显高于 IIB 期患者（1.170.40，1.210.30）表达，差异均有统计学意义（t=19.730,20.710,均P0.05）。宫颈癌放疗抵抗组 NEK2 mRNA（2.390.38），circPITX1mRNA（2.500.21）均显著高于放疗敏感组（1.830.28，0.970.28），差异有统计学意义（t=7.109,28.149,均 P 0.05）。NEK2 mRNA（48.78%（20/41）和 circPITX1mRNA54.76%（23/42）高表达组的三年总生存率显著低于NEK2 mRNA90.24%（37/41），circPITX1 mRNA低表达组 85.00%（34/40），差异均有统计学意义（2=5.118,4.890,均 P 0.05）；NEK2 mRNA（26.103.62）个月 和 circPITX1mRNA（25.873.81）个月高表达组的平均生存时间显著短于NEK2 mRNA（33.254.09）个月和circPITX1mRNA低表达组（32.024.02）个月，差异均有统计学意义（t=8.382,7.140,均 P 0.05）。FIGO 分期 A B（HR=3.754，95%CI:1.6218.694），NEK2 mRNA高表达（HR=2.874，95%CI:2.5043.497），circPITX1mRNA 高表达（HR=2.679，95%CI:2.4853.184）是宫颈癌患者预后不良的独立危险因素（均 P 均 0.05）。结论NEK2 和 circPITX1 在宫颈癌组织中表达升高,可成为放疗敏感度及预后预测的标志物。关键词：宫颈癌；中心体相关激酶 2；环状 RNA 配对同源结构域基因 1 中图分类号：R737.33；R730.43文献标识码：A文章编号:1671-7414（2023）01-027-06doi:10.3969/j.issn.1671-7414.2023.01.006Correlation between the Expression Levels of NEK2 and CircPITX1 and Radiotherapy Sensitivity and Prognosis in Cervical CancerCHEN Xiao,WU Xiao-ying,AN Ming(Department of Obstetrics and Gynecology，West China Sanya Hospital of Sichuan University，Hainan Sanya 572000，China)Abstract:ObjectiveTo explore the expression never in mitosis gene A-related kinase 2(NEK2)and circRNA paired homology domain gene 1(circPITX1)in cervical cancer tissues and their correlation with radiotherapy sensitivity and prognosis.Methods82 patients with cervical cancer who received radiotherapy in West China Sanya Hospital of Sichuan University from January 2017 to January 2018 were selected as research object.The efficacy of radiotherapy was evaluated according to the lesion shrinkage after radiotherapy,and the patients were divided into radiotherapy resistant group(n=51)and radiotherapy sensitive group(n=31).The expression of NEK2 protein in cervical cancer tissues and adjacent tissues was detected by immunohistochemistry（iIHCS）.The expression of circPITX1mRNA and NEK2 mRNA was detected by fluorescence real-time quantitative PCR（RT-qPCR）.The relationship between circPITX1 mRNA and NEK2 mRNA in cancer tissue with radiosensitivity and clinicopathological characteristics was statistically analyzed.The Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of circPITX1mRNA and NEK2 mRNA and the 3-year prognosis of 基金项目：海南省自然科学基金项目(20169426)。作者简介：陈霄（1987-），女，本科，主治医师，研究方向：妇产科疾病相关研究，E-mail：。28现代检验医学杂志第 38 卷第 1 期2023 年 1 月J Mod Lab Med,Vol.38,No.1,Jan.2023cervical cancer patients.COX regression analysis was used to analyze the risk factors of poor prognosis in patients with cervical cancer.ResultsThe positive rate of NEK2 protein expression in cervical cancer tissues 85.37%(70/82)was higher than that in adjacent tissues 12.20%(10/82),and the difference was statistically significant(2=87.857,P 0.05).The expressions of circPITX1mRNA(1.920.24)and NEK2 mRNA(2.040.31)in cancer tissues were higher than those of circPITX1 mRNA(0.810.20)and NEK2 mRNA(0.930.27)paracancerous tissues,and the differences were statistically significant(t=32.174,24.450,all P 0.05).There was a significant positive correlation between NEK2 mRNA and CircPITX1mRNA expression in cervical cancer tissue(r=0.618,P 0.05).The expressions of NEK2 mRNA(2.540.23)and circPITX1mRNA(2.330.19)in patients with FIGO stage IIIA IIIB cervical cancer were significantly higher than those in IIB stage patients(1.170.40)and CircPITX1 mRNA(1.210.30)，and the differences were statistical significance(t=19.730,20.710,all P 0.05).The NEK2 mRNA(2.390.38)and circPITX1mRNA(2.500.21)in the radiotherapy-resistant cervical cancer group were significantly higher than those in the radiosensitive group(1.830.28,0.970.28),and the differences were statistically significant(t=7.109,28.149，all P 0.05).The 3-year overall survival rate of NEK2 mRNA(48.78%(20/41),circPITX1mRNA 54.76%(23/42)high expression group was significantly lower than that of NEK2 mRNA 90.24%(37/41),and CircPITX1mRNA low expression group 85.00%(34/40),and the differences were statistically significant(2=5.118,4.890,al