TranscriptomeanalysisofChouioiacuneaYanginresponsetoavermectinstressJINXin1，2，QINDongyu1，2，LIUXinyu1，2，ZHANGTiantian1，2，RENRui1，2，GUOMeiqi1，2，LIMin1，2，ZHUGengping1，2，PANLina1，2（1.CollegeofLifeSciences，TianjinNormalUniversity，Tianjin300387，China；2.TianjinKeyLaboratoryofConservationandUtilizationofAnimalDiversity，TianjinNormalUniversity，Tianjin300387，China）Abstract：ToexplorethestresseffectofpesticideswidelyappliedinagricultureandforestryonChouioiacuneaYang，thesafetyofavermectin，whichisakindofantibioticinsecticide，onC.cuneawasassessedbythemethodoffilmbioassay.TranscriptomesequencingwasusedtoanalyzethealternationofcertaingeneexpressionofC.cuneaunderavermectinstress.Theresultsshowedthat：①BecauseofthelowtoxicityofavermectinwiththeconcentrationusedinthefieldtoC.cunea，avermectinisconsideredtobealowriskpesticide，whilehighconcentrationstresshadshort-termkillingeffectonC.cunea；②Comparedwiththecontrolgroup，atotalof805differentiallyexpressedgeneswereobtainedbytranscriptomesequencingofC.cuneaunderavermectinstresswithLC10andLC30concentrations，ofwhich33geneswereup-regulatedand772genesweredown-regulated；③ThefunctionalanalysisofGeneOntology（GO）annotationrevealedthatthecorrespondingdifferentiallyexpressedgeneswereenrichedincellprocess，metabolicprocess，membraneandmembranecomponents，molecularbindingandcatalyticactivities；④TheanalysisofKEGGmetabolicpathwaydemonstratedthattheinteractionofneuroactiveligand-receptorsandthecytochromeP450-induceddrugmetabolizingpathwayweresignificantlyenriched；⑤DetectionofcytochromeP450-relatedgenesbyRT-qPCRshowedthattheexpressionlevelsofCcCYP9AG3andCcCYP9AG4weresignificantlyhigherintheavermectinstressgroupthaninthecontrolgroup，whichindicatedthatthecytochromeP450genefamily，inparticular，theCYP9familyisinvolvedintheresponseofC.cuneatoavermectinstress.Keywords：ChouioiacuneaYang；avermectin；toxicitydetermination；stressresponse；transcriptomesequencing周氏啮小蜂对阿维菌素胁迫响应的转录组分析金欣1，2，覃东玉...
