研究报告ResearchReport重瓣铁线莲花色与花青素的关系袁明霞1宋微1*刘换换1朱骏1陈天森1丁益21江苏农林职业技术学院,句容,212400;2苏州极创澜海科技有限公司,苏州,215000*通信作者,songwei@jsafc.edu.cn摘要为探明重瓣铁线莲花色与花青素的关系，本研究采用液质联用检测技术(HPLC-MS)对4个色系重瓣铁线莲中6种主要花青素进行了定量分析。结果表明，各色系重瓣铁线莲中均含有花青素，但花青素单体含量及总量差异显著，且不同色系中优势花青素单体不同。红色系重瓣铁线莲花青素中芍药素和矢车菊素含量明显高于紫色系和蓝色系，最高可达30.7倍和13.0倍，说明芍药素和矢车菊素可能与红色着色相关。紫色系重瓣铁线莲花青素中飞燕草素含量高于红色系，但低于蓝色系；蓝色系重瓣铁线莲花青素中飞燕草素含量最高，说明飞燕草素含量可能影响着花色从紫色到蓝色的变化。供试白色系重瓣铁线莲中只检出了少许矢车菊素，未检测到其他5种花青素，说明纯白色系重瓣铁线莲中可能不含花青素物质，检出的少许矢车菊素与供试材料萼瓣端部的微红色有关。此外，红色系重瓣铁线莲的6种花青素总量远高于其他色系，说明萼瓣呈色不同与花青素总量差异有很大关系。本研究的实施为探明铁线莲属萼瓣呈色机理奠定了基础，亦为其花色育种提供了理论依据。关键词铁线莲属;重瓣;花色;花青素TheRelationshipBetweentheColorsandAnthocyaninsofClematisHybri-daswithDoublePetalsYuanMingxia1SongWei1*LiuHuanhuan1ZhuJun1ChenTiansen1DingYi21JiangsuVocationalCollegeofAgricultureandForestry,Jurong,212400;2JiangsuJichuanglanhaiTechnologyCo.Ltd.,Suzhou,215000*Correspondingauthor,songwei@jsafc.edu.cnDOI:10.13271j.mpb.021.000941AbstractInordertofindouttherelationshipbetweenthecolorsandanthocyaninsofClematishybridas(Clema-tisfloridaThunb.)withdoublepetals,6majoranthocyaninsinClematishybridaswithdoublepetalsof4colorsys-temswerequantitativelyanalyzedbyHPLC-MS.TheresultsshowedthatanthocyaninsweredetectedinallClema-tishybridas,butthemonomercontentandtotalamountofanthocyaninsweresignificantlydifferent,andthedomi-nantanthocyaninmonomerindifferentcolorsystemsweredifferent.ThecontentsofpeonidinandcyanidininredClematishybridasweresignificantlyhigherthanthoseinpurpleandblueClematishybridas,upto30.7and13.0times,indicatingthat...
