改良快速骨组织脱钙法在透射电镜样品制备中的应用王立言，邴馨，刘尚明*，王辉，陈艳雯（山东大学基础医学院实验核医学与电镜中心，济南250012）〔摘要〕目的探讨改良快速骨组织脱钙法在透射电镜样品制备中的应用效果。方法使用JYBL-Ⅱ组织脱钙液，加入电镜专用戊二醛固定液，并对渗透压等影响因素进行了有效控制，与传统脱钙法处理电镜样品后进行比较。结果改良快速骨组织脱钙法使脱钙时间由传统的2~4周缩短至24h，电镜观察骨组织超微结构完整性更好，结构更清晰。结论改良快速骨组织脱钙法在透射电镜样品制备中具有较高的应用价值，值得推广。〔关键词〕脱钙；骨组织；透射电镜〔中图分类号〕R34〔文献标识码〕ADOI：10.16705/j.cnki.1004-1850.2022.06.011Applicationofimprovedrapidbonedecalcificationinpreparationoftransmissionelec-tronmicroscopysamplesWangLiyan,BingXin,LiuShangming*,WangHui,ChenYanwen(CenterforExperimentalNuclearmedicineandElectronMicroscope,SchoolofBasicMedicalSciences,ShandongUniversity,Jinan250012,China)〔Abstract〕ObjectiveToinvestigatetheapplicationofimprovedrapidbonedecalcificationmethodinthesamplepreparationoftransmissionelectronmicroscopy.MethodsTheimprovedmethodusedJYBL-Ⅱtissuedecalcificationsolutionandaddedglutaralde-hydefixativesolutionforelectronmicroscope.Theinfluencefactorssuchasosmoticpressurewereeffectivelycontrolled.Theresultswerecomparedwiththoseoftraditionaldecalcificationmethod.ResultsTheimprovedrapidbonedecalcificationmethodshortenedthedecalcificationtimefromthetraditional2-4weeksto24hours,andtheultrastructureofbonetissueismorecompleteandclearerunderelectronmicroscopy.ConclusionTheimprovedmethodofrapidbonedecalcificationhasimportantapplicationvalueinTEMsamplepreparation.〔Keywords〕Decalcification;bonetissue;transmissionelectronmicroscope中国组织化学与细胞化学杂志CHINESEJOURNALOFHISTOCHEMISTRYANDCYTOCHEMISTRY第31卷第6期2022年12月Vol.31.No.6December.2022〔收稿日期〕2022-09-05〔修回日期〕2022-12-08〔基金项目〕山东大学实验室建设与研究管理重大项目(SY20211401)；山东大学实验室建设与管理研究项目（sy20223404）〔作者简介〕王立言，男（1975年），汉族，实验师*通讯作者(Towhomcorrespondenc...
