·实验教学·姜黄素纳米胶囊亲水改性及体外评价综合实验设计刘焕迪1，杜洁1*，张红蕾1，贾新超2，张路路3，李玮1（1.河北大学化学与材料科学学院，保定071002；2.晨光生物科技集团股份有限公司，邯郸056000；3.河北牧群生物科技有限公司，保定071002）摘要：本实验合成并纯化得到两亲性聚羟基脂肪酸酯颗粒表面结合蛋白（PhaP），将其修饰在前期制备的姜黄素纳米胶囊（Cur@PHA）表面，通过表面改性有效提高了纳米胶囊的分散性能；然后对表面改性姜黄素纳米胶囊（Cur-PhaP@PHA）的形貌、稳定性、药物释放性能及体外细胞毒性进行了表征和分析。结果表明：Cur-PhaP@PHA表面形态和球形度好，光稳定性能优，具有良好的药物缓释、控释作用，表现出更优的药物吸收和生物利用度。该综合实验设计由产业需求出发，帮助学生从产业应用、技术创新的角度分析解决问题，有利于提高学生综合创新能力。关键词：姜黄素；聚羟基脂肪酸酯；两亲性纳米胶囊；释药性能；综合性实验中图分类号：R945文献标志码：ADOI:10.12179/1672-4550.20230030ComprehensiveExperimentDesignonHydrophilicModificationandEvaluationinVitroofCurcuminNano-capsulesLIUHuandi1,DUJie1*,ZHANGHonglei1,JIAXinchao2,ZHANGLulu3,LIWei1（1.CollegeofChemistryandMaterialsScience,HebeiUniversity,Baoding071002,China;2.ChenguangBiotechGroupLimitedCo.,Ltd.,Handan056000,China;3.MuqunBiotechCo.,Ltd.,Baoding071002,China）Abstract:Inthisexperiment,amphiphilicepolyhydroxyalkanoate(PHA)particlesurfacebindingprotein(PhaP)wassynthesizedandpurified.PhaPwaseffectivelymodifiedonthesurfaceofcurcuminnanosphere(Cur@PHA),andthedispersionperformanceofnanospherewaseffectivelyimprovedbysurfacemodification.Themorphology,stability,drugreleaseandinvitrocytotoxicityofthedrugdeliverysystemofCur-PhaP@PHAwerecharacterizedandanalyzed.TheresultsshowedthatCur-PhaP@PHAhadgoodsphericity,excellentlightstability,andsustainedandcontrolledrelease.Italsoexhibitedbetterdrugabsorptionandbioavailabilitythanfreecurcumin.Thiscomprehensiveexperimentaldesignisbasedonindustrialdemand,inspiringstudentstoanalyzeandsolveproblemsfromtheperspectiveofindustrialapplicationandtechnologicalinnovation,whichisconducivetoimprovingstudents’comprehensiveinnova...
