materials-2593.pdf

Journal of Visualized ECopyright 2011 Creative Commons Attribution-NonCommercial LicensePage 1 of 1Materials List for:Detection of Post-translational Modifications on Native Intact Nucleosomes byELISABo Dai1,Farida Dahmani2,Joseph A.Cichocki3,Lindsey C.Swanson2,Theodore P.Rasmussen31Institute for Stem Cell Biology and Regenerative Medicine,Stanford University2Department of Molecular and Cell Biology,University of Connecticut3Department of Pharmaceutical Sciences,University of ConnecticutCorrespondence to:Theodore P.Rasmussen at theodore.rasmussenuconn.eduURL:http:/ NumberCommentsMicrococcal NucleaeSigma-AldrichN3755Make aliquots of 2 U/10l buffer,stored at-20CNunc MaxiSorp Microtiter PlatesFisher Scientific12-565-135Adhesive plate covers can also beordered through FisherAutomated Plate Washer1-Step TMB-ELISAsubstratePierce,Thermo Scientific34028Store at 4C pre-warm to RTbefore use or as directed on thespecification sheetMicrotiter Plate ReaderBio-RadMost colorimetric plate readermodels are suitable