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jove
materials
3418
scale
up
of
mammalian
cell
Journal of Visualized ECopyright 2011 Journal of Visualized Experiments and Corning,Inc.Page 1 of 1Materials List for:Scale-Up of Mammalian Cell Culture using a New Multilayered FlaskElizabeth J.Abraham1,Katie A.Slater1,Suparna Sanyal1,Ken Linehan1,Paula M.Flaherty1,Susan Qian11BD BiosciencesURL:http:/ NumberComments3-layer Tissue Culture-treated525cm2BD Biosciences3531435-layer Tissue Culture-treated875cm2BD Biosciences353144100ml pipetteBD Biosciences35760010 ml pipetteBD Biosciences3575515 ml aspirating pipetteBD Biosciences35750150 ml Polypropylene conical tubeBD Biosciences352070T-175 flask Tissue Culture-treatedBD Biosciences353028Gram Crystal VioletBD Biosciences212525DMEMInvitrogen11885GMEMSigma-AldrichG5154RPMI-1640ATCC30-2001MEMATCC30-2003Trypsin-EDTALonza Inc.CC-5012Fetal Bovine serumInvitrogen16000-044Tryptose Phosphate BrothSigma-AldrichT8159BHK-21 cellsSigma-Aldrich85011433Hep-G2,LnCAPATCCEcopack 2-293Clontech LaboratoriesParaformaldehydeElectron Microscopy Sciences15710Polystyrene BeadPolysciences,Inc.24628-20Bio-Profile Flex AnalyzerNova BioMedical