jove-materials-1871-dna-based-fish-species-identification-protocol.pdf

Journal of Visualized ECopyright 2010 Journal of Visualized Experiments and Agilent Technologies,Inc.Page 1 of 1Materials List for:DNA-based Fish Species Identification ProtocolRachel Formosa1,Harini Ravi1,Scott Happe1,Danielle Huffman1,Natalia Novoradovskaya1,Robert Kincaid1,Steve Garrett11Agilent TechnologiesCorrespondence to:Rachel Formosa at URL:http:/ NumberCommentsAgilent 2100 Bioanalyzer(withchip priming station and IKA vortexmixer)Agilent TechnologiesG2939AAAgilent 2100 Bioanalyzer laptop&Expert SoftwareAgilent TechnologiesG2953CAG2950CA-desktop PC&ExpertSoftwareAgilent Bioanalyzer DNA 1000Reagent Kit(part#5067-1504)Agilent Technologies5067-1504Thermal CyclerVariousVariousPCR Strip TubesAgilent Technologies401428PCR Strip CapsAgilent Technologies40142596-Well Working RackAgilent Technologies410094Fish Species Identification SystemDNA Isolation KitAgilent TechnologiesContactFish Species Identification SystemPCR-RFLP Reagent KitAgilent TechnologiesContactRFLP Matcher SoftwareAgilent TechnologiesContact100%ethanol,200 proof(USPgrade or equivalent)VariousVariousSterile,nuclease-free waterVariousVariousPipettorsVariousVariousIncubator or water bath set to 65CVariousVariousSterile glass bottle orpolypropylene tube(e.g.14-ml BDFalcon polypropylene)VariousVariousround-bottom tubes or 50-ml BDFalcon polypropylene conicaltubes)VariousVariousVortex mixerVariousVariousMicrocentrifugeVariousVariousMicrocentrifuge tubes 1.5mlVariousVariousTube rack 1.5mlVariousVariousPipette TipsVariousVarious