Cellmaxinanti mycoplasma reagent.pdf

Cellmaxin(10mg/ml),anti-mycoplasma reagent For prevention and removal of mycoplasma contamination in cell culturesOrdering InformationCellmaxin,10mg/mlCellmaxin Plus,10.5mg/mlC3314-006C3314-020C3319-006C3319-0206x1ml 20ml6x1ml 20ml Product Cat No Size Less ToxicMore CuredTO ORDERToll Free 866.417.0078Fax PO Box 454 Barker,TX 77413 U.SWWW.GenDEPOT.COM Start Small for Big Change5mg/ml Macrolide,5mg/ml Quinolone5mg/ml Macrolid,5mg/ml Quinolone500ug/ml anfifungal reagentComparision of the most common antibiotics in Mammalian Cell CultureCellmaxin,10mg/mlCellmaxin PlusPenicilli-StreptomycinAntibiotics-Antimycotics(100X)Anti-microbial spectrum Mode of action Required amount MycoplasmaGram+/Gram-BacteriaProtein synthesis DNA synthesis250ul-1ml/500ml250ul-1ml/500ml5ml/500ml5ml/500mlProtein synthesisDNA synthesisMembrane enzymeCell wall synthesisProtein systhesisCell wall synthesisProtein systhesisCell membraneGram+/Gram-BacteriaFungiMycoplasmaGram+/Gram-BacteriaFungiGram+/Gram-Bacteria10,000 units/ml Penicillin G,10,000 ug/ml Streptomycin5mg/ml Macrolide,5mg/ml Quinolone5mg/ml Macrolide,5mg/ml Quinolone500ug/ml Antifungal reagent10,000 units/ml Penicillin G,10,000 ug/ml Streptomycin25ug/ml Amphotericin BComparision of the most common anti-mycoplasma agentsCellmaxinBM-Cyclin 1/2CiprofloxacinMRA2 weeks2 weeks12-20 days1-2 weeks92%cured84%cured77%cured64%cured2%5%14%22%Treatment days Efficiency ResistanceMycoplasma species are often found in research laboratories as contamination from individuals orcontaminated cell culture medium ingredients.Mycoplasma cells are physically small-less than 1m,andtherefore they are very difficult to detect with a conventional microscope.Also Mycoplasma is a genus bacteria that lack a cell wall,without a cell wall,they are unaffected by many common antibiotics such aspenicillin or other beta-lactam antibiotics that target cell wall synthesis.Mycoplasma contamination of cell culture systems continues to present major problems for basic research and manufacturing of bioproducts.Mycoplasma contamination decreases cellular activity,retards growth rate,and consequently diminishesthe quality of date or products.Cellmaxin is a new antibiotic formulation specially desigend to protect cell culture system from mycoplasmacontamination.Cellmaxin is a unique anti-mycoplasma reagent that contains a unique combination of macrolide and quinolone.Unlike other anti-mycoplasma reagent that requires the sequential and cyclic use of two antibiotics,Cellmaxin is ready-to-use and can be added to cell culture medium directly.Furthermoretwo components in Cellmaxin act on seperate targets blocking protein synthesis and DNA replication.Therefore,Cellmaxin is more effective in eliminating mycoplasma and prevent the appearance of resistant strains.The quinolone in Cellmaxin can enter cells easily and therefore are active on both intracellular pathogens as well as free mycoplasma.For many Gram+bacteria,the target of Cellmaxin is topoisomerase IV which eukaryotic cells do not contain.Therefore Cellmaxin is less toxic than other anti-mycoplasma reagents.The working concentration of Cellmaxin varies with the organism,cell type,enviromental conditions,and growth stage.However,a concentration of 10ug/ml is active most strains of bacteria anda number of mycoplasma strains.Cellmaxin is active against mycoplasma,Gram+bacteria and Gram-bacteria at low concentration(5-20ug/ml).There is no need to add Penicillin-Sterptomycin during the Cellmaxin treatment.Cellmaxin is provided as 10mg/ml clear filtered solution.Cellmaxin plus is a combination of Cellmaxin and antifungal compounds.Cellmaxin plus is active against mycoplasma,Gram+bacteria,Gram-bacteria,and fungi.There is no need to add Penicillin-Streptomycin and amphotericin B during Cellmaxin Plus treatment.Treatment of Mycoplasma infected cell cultures:20ug/ml 1,Remove contaminated medium from culture vessels by aspiration 3,Add new medium containing Cellmaxin at the final concentration of 20ug/ml 2,Remove and replace with fresh Cellmaxin containing medium every 3-4 days for 2 weeks For maintenance of Mycoplama free culture:5ug/ml Use Cellmaxin at the final concentration of 5ug/ml to prevent Mycoplasma Storage and Stability Cellmaxin is stable for at least 18 months when stored at-20oC.Avoide multiple freeze thaw cycles to maintain optimun stability Product Use Statement These products are for RESEARCH USE ONLY.35mm plateMedium Vol2ml4ul1ml200ul20ul10ul5ml10ml100ml500mlCellmaxin Vol60mm plate100mm plate100ml flask500ml bottle35mm plateMedium Vol2ml1ul250ul50ul5ul2.5ul5ml10ml100ml500mlCellmaxin Vol60mm plate100mm plate100ml flask500ml bottle