materials-2732(2).pdf

Journal of Visualized ECopyright 2011 Creative Commons Attribution-NonCommercial LicensePage 1 of 1Materials List for:A Calcium Bioluminescence Assay for Functional Analysis of Mosquito(Aedes aegypti)and Tick(Rhipicephalus microplus)G Protein-coupledReceptorsHsiao-Ling Lu1,Cymon N.Kersch1,Suparna Taneja-Bageshwar1,2,Patricia V.Pietrantonio11Department of Entomology,Texas A&M University(TAMU)2Department of Molecular and Cellular Medicine,Texas A&M University(TAMU)Correspondence to:Patricia V.Pietrantonio at p-pietrantoniotamu.eduURL:http:/ NumberCommentsDMEMInvitrogenABCD1234CHO-K1 cellsATCCCCL-61Manassas,VA,USAF12K mediumInvitrogen21127Fetal Bovine SerumSigma-AldrichF0643Trypsin-EDTA(10 x)Invitrogen15400Antibiotic-AntimycoticInvitrogen15240Opti-MEM I Reduced SerumMediumInvitrogen31985Lipofectin ReagentInvitrogen18292-011GENETICINInvitrogen10131035MC1061/P3 UltracompInvitrogenC663-03QIAprep spin miniprep kitQiagen19064FuGENE 6 Transfection reagentRoche Group11 814 443 00196-well white thin bottommicrotitere plateCostar3610calcium-free DMEM mediaInvitrogen21068ClenterazineInvitrogenC-2944Bright-Line HemacytometerHausser ScientificHorsham,PANOVOstarBMG LabtechnologiesPrism software 4.0GraphPad Software Inc.San Diego,CA,USAT-25 and T-75 FlasksBD Biosciences353014 and 353135