jove-materials-4356.pdf

Journal of Visualized ECopyright 2012 Journal of Visualized ExperimentsPage 1 of 1Materials List for:Genome-wide Gene Deletions in Streptococcus sanguinis by High ThroughputPCRXiuchun Ge1,Ping Xu11The Philips Institute of Oral and Craniofacial Molecular Biology,Virginia Commonwealth UniversityCorrespondence to:Ping Xu at pxuvcu.eduURL:http:/ NumberCommentsPrimer F2Integrated DNA TechnologiesTGACTAACTAGGAGGAATAAATG GCTAAAATGAGAATATPrimer R2ibidCATTATTCCCTCCAGGTACTAAAA CAATTCATCCAGTPrimer F2pibidGATAAACCCAGCGAACCATTTGAPrimer P1ibidGCTTATATACCTTAGCAGGAGACAPrimer P2ibidGTATGACATTGCCTTCTGCGTCCPrimer 5 end_R1_seqibidGCCATTTATTCCTCCTAGTTAGTCAPrimer 5 end_F3_seqibidGTTTTAGTACCTGGAGGGAATAATGPrimer 5 end_R1p_seqibidCCTCAAATGGTTCGCTGGGTTTATCCSPSynprepSequence:NH2-DLRGVPNPWGWIFGR-COOHDNA PolymeraseInvitrogen11304-102Platinum Taq DNA PolymeraseHigh FidelityEcoRINew England Biolabs IncR0101SRestriction enzymeAgarAmerican BioanalyticalAB01185AgarosePromega V3125BHIBD Biosciences237500Brain Heart InfusionHorse serumFisher ScientificSH3007403Horse serumKmFisher ScientificBP906-5KanamycinTH brothBD Biosciences249240Todd Hewitt brothPureLink 96InvitrogenK310096PureLink 96 PCR purification kitQIAquickQiagen28106QIAquick PCR purification kitFilterCorning4311170.22 m polystyrene filterAnoxomat SystemMart Microbiology b.v.Anoxomat Mark IIBenchtop centrifugeThermo Scientific75004377Sorvall Legend RT Plus microplaterotorGel DocumentationUVP LLCBioDoc-It 210IncubatorFisher Scientific11-690-625DIsotempLabnets Gel XLLabnetE0160Labnets Gel XLMicrocentrifugeEppendorf22621408Microcentrifuge 5415 RMultichannel pipettesThermo Scientfic4661040,4661020Finnpipette F1Thermal CyclerApplied Biosystems Inc.N805-0200GeneAmp PCR system 9700