jove-materials-3854-preparation-mou.pdf

Journal of Visualized ECopyright 2012 Creative Commons Attribution-NonCommercial LicensePage 1 of 1Materials List for:Preparation of Mouse Embryonic Fibroblast Cells Suitable for CulturingHuman Embryonic and Induced Pluripotent Stem CellsJustyna Jozefczuk1,Katharina Drews1,James Adjaye11Department of Vertebrate Genomics,Max Planck Institute for Molecular GeneticsCorrespondence to:Justyna Jozefczuk at jagodzinmolgen.mpg.de,James Adjaye at adjayemolgen.mpg.deURL:http:/ NumberCommentsDMEM(High Glucose)Gibco,Invitrogen41966-052FBSBiochromS0115L-glutamineGibco,Invitrogen25030-024Penicillin-streptomycinGibco,Invitrogen15140-122Vacuum filter systemCorning431097500 ml,0.22 m,PASDMSOSigmaD2650Knockout DMEMGibco,Invitrogen10829-018Knockout Serum ReplacementGibco,Invitrogen10828-028Non-Essential Amino AcidsGibco,Invitrogen11140-035beta-MercaptthanolSigmaM7522PBSGibco,Invitrogen14190-169DNase ISigmaD4527Mitomycin CRoche10107409001Basic Fibroblast Growth FactorPeproTech 100-18BBiotinylated Anti-human/mouse/ratActivin A AntibodyR&D SystemsBAM3381GelatinSigmaG9391Human/Mouse/Rat Activin A MAbR&D SystemsMAB33810.05%Trypsin-EDTAGibco,Invitrogen25300-054Extra Thin Iris ScissorsFST14088-10Extra Fine Graefe ForcepsFST11150-10Pierse Fixation ForcepsFST18155-13