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Journal of Visualized ECopyright 2012 Journal of Visualized ExperimentsPage 1 of 1Materials List for:Isolation of Ribosome Bound Nascent Polypeptides in vitro to IdentifyTranslational Pause Sites Along mRNASujata S.Jha1,Anton A.Komar11Center for Gene Regulation in Health and Disease,Department of Biological,Geological and Environmental Sciences,Cleveland State UniversityCorrespondence to:Anton A.Komar at a.komarcsuohio.eduURL:http:/ NumberCommentsMEGAscript T7 High yieldTranscription KitAmbionAM1333Ribonuclease InhibitorInvitrogen15518012Trans 35S-LabelMP Biomedicals0151006Ribonuclease-AInvitrogen12091Rabbit Reticulocyte Lysate System,Nuclease TreatedPromega L4960E.coli S30 Extract System forLinear TemplatesPromega L1030CentrifugationBeckman CoulterOptima TLX UltracentrifugeStorage phosphor autoradiographyGE HealthcareTyphoon 9410 variable modeimagerSoftware for nascent polypeptideanalysisGE HealthcareImage Quant TL,v2005