jove-materials-2405-assaying-surface-e(1).pdf

Journal of Visualized ECopyright 2011 Journal of Visualized ExperimentsPage 1 of 1Materials List for:Assaying Surface Expression of Chemosensory Receptors in HeterologousCellsSandeepa Dey1,Senmiao Zhan2,Hiroaki Matsunami11Department of Molecular Genetics and Molecular Biology,Duke University2Department of Chemistry,Duke UniversityCorrespondence to:Hiroaki Matsunami at matsu004mc.duke.eduURL:http:/ NumberCommentsCover slip 22 X 22 mm(#1)Thermo Fisher Scientific,Inc.72200-11Fetal bovine serumGIBCO,by Life Technologies16000Lipofectamine 2000Invitrogen11668-019Minimal essential mediumSigma-AldrichM4655With L glutamate,Earles salt andbicarbonateMowiolCalbiochem475904ParaformaldehydeEMS19208Hanks Balanced Salt Solution(HBSS)1XGIBCO,by Life Technologies14025With calcium chloride andmagnesium chlorideHEPES Buffer SolutionGIBCO,by Life Technologies15630Phosphate Buffer Saline(PBS)Cellgro21-040-CVWithout calcium and magnesiumPoly D Lysine HydrobromideSigma-AldrichP7280Sodium AzideElectron Microscopy SciencesSX0299-1Tissue culture dish 35 X 10 mmFalcon BD353801Trypsin-EDTAGIBCO,by Life Technologies253000.05%