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SDS_PAGE胶配方表.pdf
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SDS_PAGE 配方
SDS-PAGE浓缩胶(5%Acrylamide)配方表各种组份名称各种凝胶体积所对应的各种组份的取样量1 ml2 ml3ml4 ml5ml6ml8ml10 mlH2O0.681.42.12.73.44.15.56.830%Acrylamide0.170.330.50.670.831.01.31.71.0 M Tris-HCI(pH6.8)0.130.250.380.50.630.751.01.2510%SDS0.010.020.030.040.050.060.080.110%过硫酸铵0.010.020.030.040.050.060.080.1TEMED0.0010.0020.0030.0040.0050.0060.0080.01TaKaRaBIOTECHNOLOGYCATALOGONLINEhttp:/SDS-PAGE分离胶配方表各种组份名称各种凝胶体积所对应的各种组份的取样量5 ml10 ml15ml20 ml25 ml30 ml40 ml50 ml6%GelH2O2.65.37.910.613.215.921.226.530%Acrylamide1.02.03.04.05.06.08.010.01.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%GelH2O2.34.66.99.311.513.918.523.230%Acrylamide1.32.74.05.36.78.010.713.31.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0030.0060.0090.0120.0150.0180.0240.0310%GelH2O1.94.05.97.99.911.915.919.830%Acrylamide1.73.35.06.78.310.013.316.71.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212%GelH2O1.63.34.96.68.29.913.216.530%Acrylamide2.04.06.08.010.012.016.020.01.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0215%GelHzO1.12.33.44.65.76.99.211.530%Acrylamide2.55.07.510.012.515.020.025.01.5 M Tris-HCI(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02TaKaRaBIOTECHNOLOGYCATALOGONLINEhttp:/

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