JournalofEthnopharmacology158(2014)325–330ContentslistsavailableatScienceDirectJournalofEthnopharmacologyjournalhomepage:www.elsevier.com/locate/jepEthnopharmacologicalcommunicationEvaluationofantioxidant-associatedefficacyofflavonoidextractsfromatraditionalChinesemedicineHuaJuHong(peelsofCitrusgrandis(L.)Osbeck)JianpingJianga,b,1,LetianShanb,1,ZhiyunChena,1,HaishunXuc,JianpingWanga,YuwenLiud,YaokangXiongb,naTheFirstAffiliatedHospitalofZhejiangChineseMedicalUniversity,Hangzhou310006,ChinabZhejiangChineseMedicalUniversity,Hangzhou310053,ChinacZhejiangAcademyofMedicalSciences,Hangzhou310013,ChinadHangzhouInstituteforFoodandDrugControl,Hangzhou310017,ChinaarticleinfoArticlehistory:Received23March2014Receivedinrevisedform23September2014Accepted20October2014Availableonline31October2014Keywords:Citrusgrandis(L.)OsbeckHuaJuHongAntioxidativeactivityTotalflavonoidsHPLCabstractEthnopharmacologicalrelevance:HuaJuHong(HJH,peelsofCitrusgrandis(L.)Osbeck)isapopularlyusedtraditionalChinesemedicinerecordedby“CompendiumofMateriaMedica”(BenCaoGangMu)inMingDynastyofChina(1578A.D.).Withflavonoidcomponents,HJHpossesseshypolipidemiceffectassociatedwithantioxidationaction.However,noreportwasfoundregardingtheflavonoidprofileandantioxidantactivityofHJH.Materialsandmethods:Fivepurifiedflavonoidextracts(TFCA,TFCB,TFCC,TFCDandTFCE.)wereobtainedfromHJHusingCa(OH)2andHPD-300macroporousresins,andtheirtotalflavonoidsandrepresentativeflavonoidcomponentswereanalyzed.InvitrotestsofDPPHfreeradicalscavengingactivity,reducingpower,andtotalantioxidantactivityofeachextractwereevaluated.Themosteffectiveextractwasselectedforinvivoantioxidativeevaluationusingarathyperlipemiamodel.Results:ThetotalflavonoidcontentwasvaryingamongeachHJHextractanddecreasedinanorderofTFCB4TFCD4TFCC4TFCE4TFCA.TFCB,TFCD,andTFCCcontainedmorethan50%oftotalflavonoids,thehighestcontentofwhichwasfoundinTFCB(80.7%).HPLCanalysisshowedthatthecontentsofthreeflavonoidcomponents,narirutin,naringinandneohesperidin,displayedasimilartrenda...
