农产品加工2023年第1期文章编号:1671-9646(2023)01b-0022-04第1期(总第568期)农产品加工No.12023年1月FarmProductsProcessingJan.摘要:借助超声辅助纤维素酶法对红小豆蛋白进行提取,经由单因素试验和正交试验,对纤维素酶用量、超声功率、料液比、超声时间影响提取率状况展开研究。结果表明,在试验条件下料液比对红小豆蛋白得率的因素最大,超声功率与纤维素酶用量次之,超声时间相对最弱。提取红小豆蛋白的最优工艺为料液比1∶25(g∶mL),超声功率400W,超声时间30min,纤维素酶用量250U/g。实际提取率75.38%,比常规的碱溶酸沉法(提取率为69.65%)提高了5.73%。关键词:酶超声辅助;红小豆蛋白;提取率中图分类号:TS214.9文献标志码:Adoi:10.16693/j.cnki.1671-9646(X).2023.01.038OptimizationofProteinExtractionfromAdzukiBeanbyCellulaseAssistedbyUltrasoundZHANGChunhua1,2,*ZHAIAihua1(1.HeilongjiangBayiAgriculturalUniversity,Daqing,Heilongjiang163319,China;2.FoodandPharmaceuticalEngineeringCollege,SuihuaUniversity,Suihua,Heilongjiang152061,China)Abstract:Ultrasonic-assistedcellulaseextractionofAdzukibeanproteinwasstudied,viasinglefactortestandorthogonalexperi-ments,StudiedeffectonproteinextractionrateofAdzukibeanabouttheratioofmaterialtoliquid,theultrasonicpower,durationofultrasoundandadditionofcellulose.Anditturnsout:TheorderofthefactorsaffectingtheextractionrateofAdzukibeanproteinwasmaterial-liquidratio>ultrasonicpower>cellulasecontent>ultrasonictime;Theoptimumconditionsofultrasonic-assistedcellulaseextractionofAdzukibeanproteinwereasfollows:theratioofmaterialtoliquid1∶25(g∶mL),theultrasonicpower400W,durationofultrasound30min,additionofcellulose250U/g.Theactualextractionratewas75.38%,theextractionratewas69.65%byconventionalalkalinesolutionandacidprecipitation,by5.73%。Keywords:Ultrasonic-assistedcellulasemethod;Adzukibeanprotein;extractionrate超声辅助纤维素酶法优化红小豆蛋白提取工艺研究张春华1,2,*翟爱华1(1.黑龙江八一农垦大学,黑龙江大庆163319;2.绥化学院食品与制药工程学院,黑龙江绥化152061)收稿日期:2022-02-02基金项目:绥化学院生物资源高值转化与综合利用创新团队资助项...