59工艺技术《食品工业》2023年第44卷第2期超临界CO2提取牡丹花精油及其抗氧化活性研究王春杰,张立攀*,赵梦瑶,王俊鹏,李冰,胡桂芳河南省商业科学研究所有限责任公司(郑州450000)摘要对牡丹花精油的超临界提取工艺及其抗氧化活性进行研究。在单因素试验基础上,以时间、温度、压力为影响因素,以提取率为评价指标,采用Box-Behnken响应面法设计试验对超临界CO2提取牡丹花精油工艺进行优化。结果表明,最佳提取工艺为温度38℃、压力27MPa、时间2.7h,此条件下牡丹花精油的平均提取率为2.26%,与预测值2.29%接近。抗氧化活性研究表明,牡丹花精油对DPPH自由基具有较强的清除作用,其半数清除率IC50为1.783mg/mL。关键词牡丹花;精油;超临界CO2提取;响应面法;抗氧化活性SupercriticalFluidExtractionofEssentialOilfromPeonyFlowerandItsAntioxidantActivityWANGChunjie,ZHANGLipan*,ZHAOMengyao,WANGJunpeng,LIBing,HUGuifangHenanCommerceScienceInstituteCo.,Ltd.(Zhengzhou450000)AbstractTheoptimizationofsupercriticalextractionofessentialoilfrompeonyfloweranditsantioxidantactivitywerestudied.Onthebasisofsinglefactorexperiment,choosingextractionyieldasevaluationindex,theBox-BehnkenresponsesurfacemethodwasperformedtooptimizetheextractionconditionofessentialoilfromPaeoniaflowers,withextractiontime,extractiontemperatureandextractionpressureasinfluencingfactors.Theresultsshowedthatcombinedwiththepracticaloperabilityoftheextractionprocess,extractiontemperature38℃,extractionpressure27MPa,extractiontime2.7hweredeterminedastheoptimalextractionprocess.Theaverageextractionyieldwas2.26%,whichwasclosetothetheoreticalvalueof2.29%.ThisstudycouldprovideatheoreticalreferencetoextractingessentialoilfromPaeoniaflowers.AntioxidantactivitystudiesshowedthatpeonyessentialoilexhibitedexcellentabilityofscavengingeffectonDPPHfreeradicals,withanIC50valueof1.783mg/mL.KeywordsPaeoniaflowers;essentialoil;supercriticalCO2extraction;responsesurfacemethodology;antioxidantactivity*通信作者;基金项目:河南省科学院重大科研项目聚焦项目(200111002);河南省科学院基本科研业务费(220611016);河南省科学院助推科技成果转化专项(210211032)牡丹(PaeoniasufruticosaAndr.)为毛茛科芍...