兰州大学学报（自然科学版），2023，59(3）/6月JournalofLanzhouUniversity(NaturalSciences),2023,59(3)/June基于文库筛选的方法确定SPINT3与胃腺癌转移之间的关联汪海锋，王浩浩，滕理送1.宁波市北仑区人民医院（浙江大学医学院附属第一医院北仑分院）肿瘤外科，浙江宁波3158002.浙江大学医学院附属第一医院完肿瘤外科，杭州310003摘要：通过胃腺癌肺转移模型确定SPINT3与胃癌转移之间的相关性.结果表明，SPINT3sgRNA在胃腺癌肺部转移中的表达均为阳性；SPINT3的表达与转化生长因子β1(TGFβ1)的分泌密切相关.检测SPINT3与肿瘤细胞的调亡、增殖、迁移、体内转移能力和肿瘤相关通路的关联，SPINT3在MKN-45细胞中的过表达能够促进肿瘤细胞的调亡，抑制增殖与迁移.SPINT3的抑制促进了MKN-45的体内转移能力，SPINT3的表达特异性的调节了TGFβ1通路.通过药物筛选确定了SPINT3的低表达能够被阿帕替尼和SB431542所逆转，这两种药物能够促进MKN-45SPINT3shRNA的调亡，阻断体内转移.关键词：TGFβ1结合通路；SPINT3；胃腺癌；肿瘤转移中图分类号：R735.2文献标识码：A文章编号：0455-2059(2023)03-0406-09D0I:10.13885/j.issn.0455-2059.2023.03.016AssociationbetweenSPINT3andgastricadenocarcinomametastasiswasdeterminedbylibraryscreeningWANGHai-feng',WANGHao-hao',TENGLi-song?1.DepartmentofSurgicalOncology,thePeople'sHospitalofBeilunDistrict,BeilunBranchHospitaloftheFirstAffiliatedHospital,ZhejiangUniversitySchoolofMedicine,Ningbo315800,Zhejiang,China2.DepartmentofSurgicalOncology,theFirstAffiliatedHospital,ZhejiangUniversitySchoolofMedicine,Hangzhou310003,ChinaAbstract:AcorrelationbetweenSPINT3andgastriccancermetastasiswasdeterminedviathelungme-tastasismodelofgastricadenocarcinoma,theexpressionofSPINT3sgRNAwaspositiveinlungmetas-tasisnodulesandtheexpressionofSPINT3wascloselyrelatedtothesecretionofTGFβ1.Therelation-shipwasdetectedbetweenSPINT3andapoptosis,proliferation,migration,invivometastasisandtumorrelatedpathways.TheresultsshowedthattheoverexpressionofSPINT3inMKN-45cellscouldpromotetheapoptosis,inhibitproliferationandmigrationoftumorcells,whiletheinhibitionofSPINT3promotedtheinvivometastasisabilityofMKN-45,andSPINT3specificallyregulatedtheTGFβ1pathway.T...
