Rethinking organoid technology through bioengineering.pdfVIP免费

ABDispatchDate:16.09.2020·ProofNo:804,p.1123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960616263ReviewARticlehttps://doi.org/10.1038/s41563-020-00804-41PluripotencyforOrganRegeneration,InstituteforBioengineeringofCatalonia,theBarcelonaInstituteofTechnology,Barcelona,Spain.2UniversityofBarcelona,Barcelona,Spain.3DepartmentofBiologicalEngineeringandDepartmentofMechanicalEngineering,MassachusettsInstituteofTechnology,Cambridge,MA,USA.4DepartmentofAnatomyandEmbryology,LeidenUniversityMedicalCenter,Leiden,theNetherlands.5MRCLaboratoryofMolecularBiology,CambridgeBiomedicalCampus,Cambridge,UK.6DepartmentofBiologicalEngineering,MassachusettsInstituteofTechnology,Cambridge,MA,USA.7SyntheticBiologyCenter,MassachusettsInstituteofTechnology,Cambridge,MA,USA.8CentrodeInvestigaciónBiomédicaenRedenBioingeniería,BiomaterialesyNanomedicina,Barcelona,Spain.9InstitucióCatalanadeRecercaiEstudisAvançats,Barcelona,Spain.10InstituteforBioengineeringofCatalonia,theBarcelonaInstituteofTechnology,Barcelona,Spain.11UnitatdeBiofísicaiBioenginyeria,UniversitatdeBarcelona,Barcelona,Spain.12DepartmentofBioethics,SchoolofMedicine,CaseWesternReserveUniversity,Cleveland,OH,USA.13CenterforBioethics,HarvardMedicalSchool,Boston,MA,USA.14Theseauthorscontributedequally:ElenaGarreta,RogerD.Kamm.✉e-mail:nmontserrat@ibecbarcelona.euOverthepasttwodecades,stemcellresearchhasadvancedourunderstandingofkeyaspectsoforganogenesisthroughtheexploitationoftheself-organizingpropertiesofadultstemcells(ASCs)andpluripotentstemcells(PSCs).Suchprogresseshaveledtothedevelopmentofcellcultureprocedurestogeneratemicro-andminiorgan-likestructuresondemand,theso-calledorganoids.Inparallel,theemergenceofthebioengineeringfieldisleadingtotechnologicaladvancesthatallowproperinstructiveenvironments(physicalandchemical),boostingcellularresponsestowardstheformationoforgan-specificmulticellularstructuresintheseminiorgan-likestructures.Currentmethodsrelyontra-dit...