2014;74:3043-3053.PublishedOnlineFirstMarch31,2014.CancerResPhyllisS.Y.Chong,JianbiaoZhou,Lip-LeeCheong,etal.inAcuteMyelogenousLeukemiaLEO1IsRegulatedbyPRL-3andMediatesItsOncogenicPropertiesUpdatedversion10.1158/0008-5472.CAN-13-2321doi:Accessthemostrecentversionofthisarticleat:MaterialSupplementaryhttp://cancerres.aacrjournals.org/content/suppl/2014/04/01/0008-5472.CAN-13-2321.DC1.htmlAccessthemostrecentsupplementalmaterialat:CitedArticleshttp://cancerres.aacrjournals.org/content/74/11/3043.full.html#ref-list-1Thisarticlecitesby32articles,14ofwhichyoucanaccessforfreeat:E-mailalertsrelatedtothisarticleorjournal.Signuptoreceivefreeemail-alertsSubscriptionsReprintsand.pubs@aacr.orgToorderreprintsofthisarticleortosubscribetothejournal,contacttheAACRPublicationsDepartmentatPermissions.permissions@aacr.orgTorequestpermissiontore-useallorpartofthisarticle,contacttheAACRPublicationsDepartmentatonSeptember23,2014.©2014AmericanAssociationforCancerResearch.cancerres.aacrjournals.orgDownloadedfromPublishedOnlineFirstMarch31,2014;DOI:10.1158/0008-5472.CAN-13-2321onSeptember23,2014.©2014AmericanAssociationforCancerResearch.cancerres.aacrjournals.orgDownloadedfromPublishedOnlineFirstMarch31,2014;DOI:10.1158/0008-5472.CAN-13-2321MolecularandCellularPathobiologyLEO1IsRegulatedbyPRL-3andMediatesItsOncogenicPropertiesinAcuteMyelogenousLeukemiaPhyllisS.Y.Chong1,JianbiaoZhou1,Lip-LeeCheong2,Shaw-ChengLiu1,JingruQian3,TiannanGuo3,SiuKwanSze3,QiZeng4,andWeeJooChng1,2,5AbstractPRL-3,anoncogenicdual-specificityphosphatase,isoverexpressedin50%ofacutemyelogenousleukemia(AML)andassociatedwithpoorsurvival.WefoundthatstableexpressionofPRL-3conferscytokineindependenceandgrowthadvantageofAMLcells.However,howPRL-3mediatesthesefunctionsinAMLisnotknown.TocomprehensivelyscreenforPRL3-regulatedproteinsinAML,weperformedSILAC-basedquantitativeproteomicsanalysisanddiscovered398significantlyperturbedproteinsafterPRL-3over-expression.WeshowthatLeo1,acomponentofRNApolymeraseII–associatedfactor(PAF)com...
