ChineseJournalofNeuroanatomy志杂解部经神2023,39(3):269~274皮素通过抑制氧化应激反应改善脑出血小鼠神经功能廖玉慧，黄娟，王振华，王旭，陆蔚天*（重庆医科大学基础医学院人体解部学教研室，重庆400016）[摘要]目的：探讨皮素（quercetin）对脑出血（ICH）小鼠氧化应激及神经功能的影响。方法：建立小鼠脑出血模型后通过改良Garcia评分和转角试验评估各组小鼠神经功能；通过脑含水量、脑血红蛋白含量检测和苏木精-伊红（HE）染色染色评估各组小鼠大脑受损程度与组织形态变化；通过WesternBlot检测各组小鼠核因子E2相关因子2（Nrf2）和NADPH氧化酶4（Nox4）蛋白的表达水平；通过超氧化物阴离子荧光探针（DHE）和过氧化氢(H,O2）检测各组小鼠的氧化应激水平。结果：与假手术组相比，脑出血组小鼠神经功能受损，脑含水量和血红蛋白含量升高,脑组织病理结构损伤,Nrf2表达无明显变化,Nox4表达升高，氧化应激水平升高；与脑出血组相比，皮素组小鼠的神经功能障碍减轻,脑含水量和血红蛋白含量降低，脑组织病理结构损伤减轻,Nrf2表达升高,Nox4表达降低，氧化应激水平降低。结论：皮素对脑出血小鼠的神经功能具有保护作用，可能通过抑制Nox4表达水平，增强Nrf2表达水平以降低氧化应激损伤来实现。[关键词]皮素；脑出血；氧化应激；神经保护；小鼠D0I:10.16557/j.cnki.1000-7547.2023.03.003QuercetinimprovesneurologicaldysfunctioninintracerebralhemorrhagemicebyinhibitingoxidativestressresponseLIAOYuhui,HUANGJuan,WANGZhenhua,WANGZhixu,LUWeitian(DepartmentofHumanAnatomy,InstituteofBasicMedicalCollege,ChongqingMedicalUniversity,Chongqing400016,China)[Abstract]Objective:Toexploretheeffectsofquercetinonoxidativestressresponseandneurologicalfunctionandtheregulatorymechanisminintracerebralhemorrhage(ICH)mice.Methods:Themodelsofintracerebralhemorrhageinmicewereestablishedbyautologousbloodinjection.TheneurologicalfunctionofmicewereassessedbymodifiedGarciascoreandcornerturntest;thedegreeofbraindamageandhistomorphologicalchangeswereassessedbybrainwatercontent,brainhemoglobincontentdetectionandhematoxylinandeosin(HE)stain;theexpressionofnuclearfac-torerythroid2-relatedfactor2(Nrf2)andNADPHoxidase4(Nox4)wasdetectedbyWesternBlot;andtheoxidativestresslevelsweredetectedbydihydroethidiu...
