中国国境卫生检疫杂志2023年2月第46卷第1期ChineseFrontierHealthQuarantineFeb.2023，Vol46，No.1ComparisonofthestabilityofmatrixsolutionofHBsAgweaklypositivequalitycontrolserumLUQin，SUJinkun，CHENJia，LIXueming，QIPeiyan，MINGHongyanGuangzhouInternationalTravelHealthcareCenter，Guangzhou，Guangdong510635，ChinaAbstract：ObjectiveToselectdifferentmatricestopreparehepatitisBsurfaceantigen（HBsAg）indoorqualitycontrolserumforELISA，andtofindastablematrixsolutionforthepreparationofHBsAgweaklypositivequalitycontrolserum.MethodsAserummixtureofnegativeHBsAg，anti-HIV，anti-HCVandanti-treponemalwascollectedasnegativeserum（HBV-A），fourmatricesincludingnegativeHBVserum（HBsAg，HBsAb，HBeAg，HBeAg，HBeAb，HBcAb）andnegativeforanti-HIV，anti-HCV，anti-treponemalwerecollectedasantibody-negativeserum（HBV-B），aswellasfourmatrices，humanalbumin（HBV-C）andfetalbovineserum（HBV-D）wereused.Accordingto1∶1000，1∶2000，1∶4000，1∶8000，1∶16000，1：32000，1：64000dilutedHBsAgpositiveserum，adilutionseriesofdifferentmatriceswerepreparedtodetectHBsAgbyELISAmethod.2-4timesofthecut-offvaluewasconfirmedasthetargetS/COvalue.Thefourmatrixdilutionseriesstoredat4°Cfor1，3，5，7，9，11d，1monthand2months，targetS/COvaluecorrespondstothestabilityoftheconcentrationwerecompared.Thedilutionseriespreparedwiththreeselectedmorestablematriceswereobservedfor5weeksat4℃and6monthsat-20℃，andthecorrespondingconcentrationoftargetS/COvalueswascompared，andthestabilityofthequalitycontrolserumpreparedwiththethreeselectedmatriceswascomparedfor15monthsat-20℃.ResultsTheHBsAgdilutionseriespreparedwithHBV-Amatrixhadpoorstability，andtheconcentrationcorrespondingtothetargetS/COvaluewasreducedby10afterwhenstoredat4℃for1week.Dilutionseriespreparedassubstrateswereplacedat4℃for5weeksand-20℃for6months，withstableconcentrationscorrespondingtotargetS/COvalues.Thequalitycontrolserumpreparedbythethreeselectedmatriceswasplacedat-20℃for15months，andthestabilitydifferencewasnotsignificant（P>0.05），thestabilitywas...
