L-苯丙氨酸的氨基功能化γ...2O_3磁固相萃取（英文）_秦铭杉.pdf

南 开 大 学 学 报（自然科学版）Acta Scientiarum Naturalium Universitatis NankaiensisVol.561Feb.2023第56卷第1期2023年2月秦铭杉等：L-苯丙氨酸的氨基功能化-Fe2O3磁固相萃取Article ID:0465-7942-(2023)01-0102-10Magnetic Solid Phase Extraction of L-phenylalanineby Amino-functionalized-Fe2O3Qin Mingshan1,Zhu Xinyi1,Wei Dongwei1,Song Qiqi1,Jin Tianming2,Li Cun1,Yang Linyan1(1.Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of AnimalScience and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;2.Tianjin Academy of Agricultural Sciences,Tianjin,300192,China)Abstract:Four aminogenized nanoparticles were prepared as follows:-Fe2O3SiO2-cysteamine,-Fe2O3SiO2-APTMS,-Fe2O3SiO2-PEI,-Fe2O3SiO2-G1.0.Magnetic solid phase extraction experi-ments of L-phenylalanine have been carried out at a concentration of 2.510-6mol/L,different extractiontimes and pH gradients.The lower fluorescence intensity of supernatant indicated the better extract effects.As for-Fe2O3SiO2-G1.0 nanoparticles,the best adsorption was obtained at pH=5,while the adsorptiontime was 8 h.As for-Fe2O3SiO2-PEI nanoparticles,less adsorption than above could be obtained atpH=3 and 0.5 h.-Fe2O3SiO2-PEI nanoparticles took less time to extract L-phenylalanine.Functional-ized-Fe2O3nanoparticles are promising for solid-phase extraction of L-phenylalanine.Keyword:Fe;surface chemistry;IR;TEM;XPS;absorptionCLC number:O658.2Document code:A0IntroductionIn the research and applications of new nanoparticles,magnetic nanoparticles are widely used be-cause of their optical,electrical,magnetic,thermal and catalytic properties.Magnetic nanoparticlesare a kind of nanoparticles which can be manipulated by magnetic fields.This kind of particle usual-ly contains two components.The core is a magnetic material,usually iron,nickel,and cobalt.Thesurface is other materials with functionalities to improve stability,including carbon materials,ionicliquids,Metal-Organic Frameworks,and other polymeric materials.While nanoparticles were lessthan 1m in diameter(typically 5-500 nm)and larger beads were 0.5-500 m.In diameter A groupof magnetic nanoparticles with a diameter of 50-200 nm composed of many individual magneticnanoparticles is called a magnetic nanoparticle cluster,which is the basis for its further magnetic as-Received date：2021-10-25Foundation item：Supported by Outstanding Research Achievement Award Project of Tianjin Agricultural Univer-sity,the Open Topic of Guangxi Key Laboratory for the Chemistry and Molecular Engineer-ing of Medicinal Resources(CMEMR2016-B12),the National Natural Science Foundation ofChina(21875117,31572492),theMunicipal-levelInnovativeandEntrepreneurialTrainingPlan for College Students(202210061109),the Veterinary Bitechnology Scientific Research In-novation Team of Tianjin,China(TD13-5091),the Veterinary“Innovative Talent People ofYoung and Middle Age Key Members Project”of Tianjin,the Natural Science Foundation ofTianjin,China(18JCYBJC30100,19JCQNJC13700)Biography：Qin Mingshan(1995-),male,native place:Heilongjiang Shuangyashan,Master.Corresponding author：Yang Linyan(1982-),female,native place:Hebei Jinzhou,lecturer,direction of re-search:Nanoscale drug carrie.E-mail:y_sembly into a magnetic nano-chain1-3.Magnetic nanoparticles are the core and foundation of magnet-ic solid phase extraction technology.By changing the functional part of the surface,the effective ex-traction of different target analytes can be realized4.Among them,polyamidoamine(PAMAM)dendrimers have the following structural characteristics:accurate molecular structure;high geometric symmetry,a large number of functional groups that canbe modified,and there is a cavity in the molecule.The relative molecular mass is controllable andthe molecule itself is nanometer size.Based on these structural characteristics,dendritic macromole-cules have unique properties:good monodispersity,high stability,large surface area and high activity.Hence,PAMAM dendrimers with magnetic nanoparticles as the core can be prepared as magneticadsorbents5-6.Magnetic solid phase extraction(MSPE)is a solid phase extraction technology with magnetic ormagnetized materials as adsorbent7.During the MSPE process,the magnetic adsorbent is not direct-ly filled into the adsorption column,but is added to the solution or suspension of the sample to ad-sorb the target analyte to the dispersed magnetic adsorbent surface.Under the action of externalmagnetic field,the target analyte migrates with the adsorbent and elutes the measured substancethrough the appropriate solvent to separate from the matrix of the sample.Compared with the con-ventional solid phase extraction materials,the magnetic extraction material has a larger specific sur-face area and does not need a cumbersome column loading process.The sample solution can be add-ed directly for extraction8.In the process of extraction,it can be completely dispersed into the sam-ple solution and in full contact with the measured substance,so only a short equilibrium time