东南园艺2023,11(1):11-17SoutheastHorticultureDOI:10.20023/j.cnki.2095-5774.2023.01.00364个蓝莓品种的ISSR分析张立杰J谢丽雪J张小艳1,李韬",黄立新$C福建省农业科学院果树研究所,福建福州350013;2邵武市农业农村局,福建南平354000)摘要:为明确不同蓝莓品种间的遗传关系,利用ISSR标记对64个蓝莓品种进行DNA指纹分析,构建蓝莓品种的指纹图谱数据库。从60条ISSR引物中筛选出9条重复性稳定的多态性引物,共扩增出条带161条,其中多态性条带159条,平均多态百分率达98.8%,引物UBC841可将64个蓝莓品种区分开。利用扩增结果进行聚类分析,在相似系数0.568的水平可将试材分为3个类群,并对其遗传关系进行探讨。关键词:蓝莓;品种;ISSR;指纹图谱;遗传关系中图分类号:S682.31文献标识码:A文章编号:2095-5774(2023)01-0011-07ISSRAnalysisof64BlueberryCultivarsZhangLijie1,XieLixue1,ZhangXiaoyan1,LiTao1*,HuangLixin2(〔FruitResearchInstitute,FujianAcademyofAgriculturalSciences,Fuzhou,Fujian350013,China;2ShaowuMunicipalBureauofAgricultureandRuralAffairs,Nanping,Fujian354000,China)Abstract:Inordertoclarifygeneticrelationshipofdifferentcultivarsofblueberry,theinter-simplesequencerepeat(ISSR)methodwasappliedtoanalyzetheDNAfingerprintingof64blueberrycultivars,andafingerprintsdatabasewasconstructed.NineISSRprimers,withhighpolymorphismsandgoodrepeatability,wereselectedfrom60ISSRprimers.Total161DNAbandswereamplified,159ofwhichwerepolymorphic,theaveragepolymorphismpercentagewas98.8%.ThesevarietiescouldbesatisfactorilydistinguishedbyasingleISSRprimerUBC841.UPGMAalgorithmshowedthat64cultivarscouldbeclusteredinto3groupsusingageneticsimilaritycoefficientthresholdof0.568,andtheirgeneticrelationshipswasevaluated.Keywords:Blueberry;Cultivars;ISSR;DNAfingerprinting;Geneticrelationships蓝莓,又名越橘、蓝浆果,为杜鹃花科(Ericaceae)越橘属(Vaccinium)植物。蓝莓被喻为世界第三代水果,可果实鲜食、加工或作为高级化妆品原料。蓝莓因其集约化、精细化栽培、收稿日期:2022-12-01基金项目:福建省属公益类科研院所基本科研专项(2020R10280011,2019R1028-1)作者简介:*为通讯作者,李韬(1969-),男,副研究员,主要从事小浆果种质资源与栽培育种研究,E-mail:Leetao...