Aug.2023CHINAFOODSAFETY115分析检测UPLC-MS/MS法测定膨化食品中环己基氨基磺酸钠的含量汪强1，刘燕1，王双寿2，唐文屹1，秦雅丽1，张月松1（1.马鞍山市食品药品检验和药品不良反应监测中心，安徽马鞍山243000；2.安徽工业大学，安徽马鞍山243000）摘要：建立了一种高效、简便、准确的测定膨化食品中环己基氨基磺酸钠的超高效液相串联三重四极杆质谱法。样品以甲醇+水（1+1）提取，以亚铁氰化钾溶液和乙酸锌溶液为沉淀剂，经过涡旋振荡、超声提取以及离心之后，取上清液过滤膜上机。采用C18色谱柱进行分离，MRM负离子模式检测，基质外标定量。结果表明，环己基氨基磺酸钠在0～200ng·mL-1线性关系良好，相关系数＞0.999，该方法检出限为0.03mg·kg-1，定量限为0.10mg·kg-1。在0.200mg·kg-1、0.500mg·kg-1加标水平下，回收率分别为84.0%～95.0%、96.2%～99.2%，相对标准偏差均＜10%。本方法快捷、灵敏、准确，可用于膨化食品中环己基氨基磺酸钠的检测。关键词：环己基氨基磺酸钠；膨化食品；超高效液相串联三重四极杆质谱法DeterminationofCyclamateinPuffedFoodbyUltraHighPerformanceLiquidChromatography-TandemTripleQuadrupleMassSpectrometryWANGQiang1,LIUYan1,WANGShuangshou2,TANGWenyi1,QINYali1,ZHANGYuesong1(1.MaanshanInstituteforFoodandDrugControlandAdverseDrugReaction,Maanshan243000,China;2.AnhuiUniversityofTechnology,Maanshan243000,China)Abstract:Toestablishaefficient,simpleandaccuratemethodforthedeterminationofcyclamateinpuffedfoodbyultra-performanceliquidchromatography-triplequadr-upoletandemmassspectrometry.Thesamplewasextractedwithmethanol+water(1+1),andpotassiumferricyanidesolutionandzincacetatesolutionwereusedasprecipitants,extractedbyvortexandultrasound,centrifugedandthesupernatantwastakenoverthemembraneandputonthemachine.TheseparationwascarriedoutonaC18column,detectedbyMRMnegativeionmodeandquantifiedbymatrixexternalstandard.Theresultsshowedthatthecorrelationcoefficientofsodiumcyclamateintherangeof0～200ng·mL-1wasgoodwiththecorrelationcoefficients＞0.999.Thelimitofdetectionofthemethodwas0.03mg·kg-1,andthelimitofquantificationwas0.10mg·kg-1.Therecoveriesofsodiumcyclamateatthespikedlevelsof0.200mg·kg-1,0.500mg·kg-1were84.0%～95.0...
