JournalofLanzhouUniversity(NaturalSciences),2023,59(3)/June兰州大学学报(自然科学版),2023,59(3)/6月不同磷酸盐供应水平下植物细胞胞内及胞外ATP水平的变化及一氧化氮的调节作用王娟娟,达晓伟,王馨,庞海龙2,贾凌云2,冯汉青1.21.西北师范大学生命科学学院,兰州7300702.西北师范大学新农村发展研究院,兰州730070摘要:以BY-2烟草悬浮细胞为实验材料,探究在不同磷酸盐供应水平下细胞内ATP(iATP)、细胞外ATP(eATP)水平的变化以及NO在其中的调节作用.结果表明,随着磷酸盐供应水平的增加,iATP水平呈现上升趋势;伴随着iATP的上升,eATP也呈现相似的变化.与ATP的变化不同,随着磷酸盐供应水平的增加NO水平呈现下降趋势.在正常水平磷酸盐供应下,NO供应剂可增加iATP和eATP水平;NO合成抑制剂降低了iATP和eATP水平.在较低和较高水平的磷酸盐供应下,NO供应剂和合成抑制剂对于iATP和eATP水平的影响呈现与正常水平磷酸盐供应下不同的调节效应,表明磷酸盐供应水平能够影响iATP和eATP的水平;NO对细胞ATP水平具有调节作用,其调节效应与环境磷酸盐供应水平有关.关键词:磷酸盐;一氧化氮;胞外ATP;胞内ATP中图分类号:Q942文献标识码:A文章编号:0455-2059(2023)03-0346-05D0I:10.13885/j.issn.0455-2059.2023.03.008ChangesinintracellularandextracellularATPlevelsinplantcellsunderdifferentphosphatesupplylevelsandregulationofnitricoxideWANGJuan-juan',DAXiao-wei,WANGXin',PANGHai-long*,JIALing-yun'2,FENGHan-qing.-21.CollegeofLifeScience,NorthwestNormalUniversity,Lanzhou730070,China2.NewRuralDevelopmentResearchInstitute,NorthwestNormalUniversity,Lanzhou730070,ChinaAbstract:BY-2tobaccosuspensioncellswereusedasexperimentalmaterialstoinvestigatethechangesintheintracellularATP(iATP)andextracellularATP(eATP)levelsunderdifferentlevelsofphosphatesupplyandtheregulatoryroleofnitricoxideinthisprocess.TheresultsshowedthatthelevelofiATPin-creasedwiththeincreasinglevelofphosphatesupply,whichwasfollowedbyanincreaseofeATPlevelanddecreaseofthecontentofNO.Underanormallevelofphosphatesupply,theapplicationofNOdo-norincreasedthelevelsofiATPandeATP,whiletheinhibitorofNOsynthesisreducedthatofiATPandeATP.Undertheconditionofeitheralowerorhighlevelofphosphatesupply,theeffectsof...