554研究论著新医学2023年8月第54卷第8期microRNA-124过表达对肺动脉高压大鼠肺血管重构的影响朱键陈天天刘智勇郑常龙【摘要】目的研究过表达微RNA-124（miR-124）对肺动脉高压大鼠肺血管重构的影响及初步探索其可能机制。方法使用野百合碱（MCT）经腹腔注射建立大鼠肺动脉高压模型，气管内滴注腺相关病毒（AAV）介导的miR-124进行干预，记录大鼠心脏右室收缩压（RVSP）、平均动脉压和心率，实时荧光定量PCR法检测大鼠肺组织中miR-124的表达，vanGieson染色观察肺内小动脉的重构程度并计算血管外径在25~50μm以及51~100μm的肺小动脉的中膜/外径比值，蛋白免疫印迹法检测肺组织中IL-6与p-STAT3的蛋白水平。结果miR-124在肺动脉高压大鼠肺组织中呈低表达；过表达miR-124可导致肺动脉高压大鼠RVSP下降，肺内小动脉中膜/外径明显降低，肺组织中IL-6和p-STAT3的表达下调。结论AAV介导的miR-124过表达可明显减轻肺动脉高压的大鼠的RVSP以及肺血管重构，其机制可能与下调IL-6和p-STAT3的表达有关。【关键词】微核糖核酸-124；肺血管重构；肺动脉高压EffectofoverexpressionofmicroRNA-124onpulmonaryvascularremodelinginratswithpulmonaryarterialhypertensionZhuJian△，ChenTiantian，LiuZhiyong，ZhengChanglong.△DepartmentofEmergency，theThirdAffiliatedHospitalofSunYat-senUniversity，Guangzhou510630，ChinaCorrespondingauthor，ZhengChanglong，E-mail:zhchl5@mail.sysu.edu.cn【Abstract】ObjectiveToevaluatetheeffectofoverexpressionofmicroRNA-124（miR-124）onpulmonaryvascularremodeling，andtounravelthepotentialmechanisminratswithpulmonaryarterialhypertension（PAH）.MethodsInthemonocrotaline（MCT）-inducedPAHratmodels，intratrachealinfusionofmiR-124carriedbyadeno-associatedvirus（AAV）wasperformed.Therightventricularsystolicbloodpressure（RVSP），meansystolicarterialpressureandheartratewererecorded.TheexpressionlevelofmiR-124inlungtissuesofratswasdetectedbyreal-timequantitativePCR.ThehistologicalmorphologyofpulmonaryarterioleswasobservedbyvanGiesonstaining.Theratiosofmediumtooutsidediameterofpulmonaryarterioleswithvesseldiametersin25-50μmand51-100μmwerecalculated.TheexpressionlevelsofIL-6andp-STAT3proteininlungtissuesweredetectedbyWesternblot.ResultsTheexpression...
