蓖麻花粉活力检测方法及其特性研究贾娟霞,韩雯毓,乔文杰,莫德乐吐,包双喜,刘洋,曹文强,何智彪(通辽市农牧科学研究所,内蒙古通辽028015)[摘要]以通蓖5号、091078为试材,通过花粉离体萌发、染色法、田间授粉测定法测花粉活力,探讨蓖麻花粉检测最适方法,研究温度、湿度、时间对蓖麻花粉活力的影响。结果表明:091078在蔗糖15%+硼酸0.010%的培养基、35℃的培养温度下,花粉萌发率最高(75.40%),通蓖5号在蔗糖10%+硼酸0.005%的培养基、30℃的培养温度下,花粉萌发率最高(82.08%);离体萌发试验测定的数据最为精准,但复杂耗时长,TTC染色法相对简单、迅速、方便,田间授粉测定只能定性而不能定量;蓖麻花粉萌发的最适温度为30~35℃,最适湿度为45%~55%,在散粉后的3~4h时,花粉活力最大,在12~13h后活力降低或丧失。[关键词]蓖麻;花粉活力;花粉萌发[中图分类号]S565.6[文献标志码]A[文章编号]1671-0185(2023)04-0335-05StudyontheDetectionMethodandCharacteristicsofCastorPollenViabilityJIAJuan-xia,HANWen-yu,QIAOWen-jie,Modeletu,BAOShuang-xi,LIUYang,CAOWen-qiang,HEZhi-biao(TongliaoInstituteofAgriculturalandAnimalHusbandryScience,Tongliao028015,China)Abstract:Castorpollenviabilitywasdeterminedbymeansofpollengerminationinvitro,stainingandfieldpolli⁃nation,andtheeffectsoftemperature,humidityandtimeoncastorpollenviabilitywerestudied.Resultsshowedthatthepollengerminationrateof091078wasthehighestby75.40%undertheculturemediumof15%sucroseand0.010%boricacidat35℃,andthepollengerminationrateofTongbi5wasthehighestby82.08%undertheculturemediumof10%sucroseand0.005%boricacidat30℃.Invitrogerminationtesthasthemostaccu⁃ratedata,butitiscomplicatedandtime-consuming.TTCmatchingmethodissimple,rapidandconvenient,andfieldpollinationtestcanonlybequalitativebutnotquantitative.Theoptimumtemperatureforgerminationofcas⁃torpollenwas30-35℃andtheoptimumhumiditywas45%-55%.Thepollenviabilityreachedthemaximumat3-4hafterpowderscattering,anddecreasedorlostat12-13h.Keywords:Castor;Pollenvitality;Pollengermination蓖麻(RicinuscommunisL.)作为世界十大油料作物之一,被誉为极具开发潜力又可再生的“绿色石油”[1]。随着蓖麻...