ISSN1672-4305CN12-1352/N实验室科学LABORATORYSCIENCE第26卷第3期2023年6月Vol.26No.3Jun.2023GC-MS在实验教学中的应用周蕾,王文明,胡坪,张文清,钱俊红,刚洪泽,杨世忠(华东理工大学化学与分子工程学院,上海200237)摘要:为培养化学类专业本科生的现代重要仪器使用和数据分析能力,将气相色谱-质谱联用仪(GC-MS)结合全扫描(Scan)/选择离子监测(SIM)模式分析应用于本科优班的实验教学。通过提出问题进行思考式预习,有针对性地引导学生提前进行文献检索和基本原理学习。设计了针对挥发性脂肪酸(VFA)定性/定量分析的实验,对VFA(C1~C4)丁酯化产物进行Scan模式定性分析、SIM模式绘制标准曲线以定量分析,最终测定实际样品中VFA的含量。使学生充分了解了仪器分析的常规思路和方法,加深了对仪器构造、原理的理解,掌握了仪器操作、样品前处理等实验技能,并培养了科学思维和创新探索能力。关键词:气质联用;本科实验教学;全扫描;选择离子监测;挥发性脂肪酸中图分类号:O657文献标识码:Bdoi:10.3969/j.issn.1672-4305.2023.03.006ApplicationofGC-MSinexperimentalteachingZHOULei,WANGWenming,HUPing,ZHANGWenqing,QIANJunhong,GANGHongze,YANGShizhong(SchoolofChemistryandMolecularEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,China)Abstract:Inordertocultivatetheabilityofutilizingmoderninstrumentsandanalyzingdataforunder-graduatesmajoringinchemistry,gaschromatography-massspectrometry(GC-MS)combinedwithScan/selectiveionmonitoring(SIM)modeanalysiswasappliedtoinstrumentalanalysisexperimenttargetingthequalitativeandquantitativeanalysisofvolatilefattyacids(VFA).Byraisingquestions,studentswereledtocarryouttargetedliteraturesearchandbasicprinciplelearninginadvance,inor-dertopreviewtheexperimentalcontentinathinkingway.Butyl-esterificationproductsofVFA(C1~C4)werequalitativelyandquantitativelyanalyzedwithScanandSIMmode,whichwerealsousedtoanalyzethecontentsofVFAinoilfieldsamples.Thiscourseenablesstudentstounderstandtheconven-tionalideasandmethodsofinstrumentalanalysis,deepentheunderstandingofinstrumentstructureandprinciple,masterexperimentalskillssuchasinstrumentoperationandsamplepreparation,andalsocultivatetheabilityofscientificthinkingandinnovativeexpl...
