直肠瘾癌558·ClinJMedOffic,Jun,2023临床军医杂志2023年6月第51卷第6期H3型组胺受体在结直肠癌中作用研究边振远，王鑫宇，张成北部战区总医院普通外科，辽宁沈阳110016[摘要]目的探讨H3型组胺受体在结直肠癌中的作用。方法去人结直肠癌组织和癌旁组织样本来自于北部战区总医院普通外科。将人结肠癌细胞HCT-116细胞分人siCtrl组和siH3HR组。采用WesternBlot检测结直肠癌和癌旁组织中的H3HR表达水平；使用H3HR干涉片段对结直肠癌HCT-116细胞进行处理，采用EDU、划痕实验检测H3HR干涉对siH3HR组、siCtrl组细胞增殖能力的影响及对HCT-116细胞迁移能力的影响。结果果在10对结直肠癌和癌旁组织中，结直肠癌组织中H3HR表达水平高于癌旁组织。H3HR干涉后，siH3HR组细胞增殖能力低于siCtrl组（P<0.05）。干涉降低H3HR表达后,HCT-116细胞迁移能力下降(P<0.05）。结论H3HR与结直肠癌细胞的增殖和迁移相关。[关键词]结直肠癌；H3型组胺受体；增殖；迁移中图分类号：R735.3doi:10.16680/j.1671-3826.2023.06.03文章编号：1671-3826(2023)06-0558-03TheroleofH3histaminereceptorincolorectalcancerBIANZhen-yuan,WANGXin-yu,ZHANGCheng(DepartmentofGeneralSurgery,GeneralHospitalofNorthernTheaterCommand,Shenyang110016,China)Abstract:ObjectiveToinvestigatetheroleofH3histaminereceptor(H3HR)incolorectalcancer.MethodsHumancolorectalcancertissueandparacancertissuesampleswerecollectedfromGeneralHospitalofNorthernTheaterCommand.HumancoloncancercellsHCT-116weredividedintosiCtrlgroupandsiH3HRgroup.WesternBlotwasusedtodetecttheexpressionofH3HRincolorectalcancerandadjacenttissues.ColorectalcancerHCT-116cellsweretreatedwithH3HRinterferencefragments.EDUandscratchtestswereusedtodetecttheeffectsofH3HRinterferenceoncellproliferationandmigrationofHCT-116cellsinsiH3HRgroupandsiCtrlgroup.ResultsIn10pairsofcolorectalcancerandparacancertissues,theexpressionlevelofH3HRincolorectalcancertissueswashigherthanthatinparacancertissues.AfterH3HRinterference,thecellproliferationabilityofsiH3HRgroupwaslowerthanthatofsiCtrlgroup(P<0.05).ThemigrationabilityofHCT-116cellswasdecreasedafterinterferencedecreasedH3HRexpression(P
