Citation:Bramley,H.;Ranawana,S.R.W.M.C.J.K.;Palta,J.A.;Stefanova,K.;Siddique,K.H.M.TranspirationalLeafCoolingEffectDidNotContributeEquallytoBiomassRetentioninWheatGenotypesunderHighTemperature.Plants2022,11,2174.https://doi.org/10.3390/plants11162174AcademicEditor:DanielaTronoReceived:7July2022Accepted:18August2022Published:21August2022Publisher’sNote:MDPIstaysneutralwithregardtojurisdictionalclaimsinpublishedmapsandinstitutionalaffil-iations.Copyright:©2022bytheauthors.LicenseeMDPI,Basel,Switzerland.ThisarticleisanopenaccessarticledistributedunderthetermsandconditionsoftheCreativeCommonsAttribution(CCBY)license(https://creativecommons.org/licenses/by/4.0/).plantsArticleTranspirationalLeafCoolingEffectDidNotContributeEquallytoBiomassRetentioninWheatGenotypesunderHighTemperatureHelenBramley1,2,*,S.R.W.M.ChandimaJ.K.Ranawana1,3,*,JairoA.Palta1,4,KatiaStefanova1andKadambotH.M.Siddique11TheUWAInstituteofAgriculture,TheUniversityofWesternAustralia,Perth,WA6001,Australia2PlantBreedingInstitute,SchoolofLifeandEnvironmentalSciences,TheUniversityofSydney,Narrabri,NSW2390,Australia3DepartmentofExportAgriculture,FacultyofAnimalScienceandExportAgriculture,UvaWellassaUniversity,Badulla90000,SriLanka4CSIROAgriculture,PrivateBagNo.5,Wembley,WA6913,Australia*Correspondence:helbramley@gmail.com(H.B.);chandima@uwu.ac.lk(S.R.W.M.C.J.K.R.)Abstract:Hightemperatureandwaterdeficitarethemostcriticalyield-limitingenvironmentalfactorsforwheatinrainfedenvironments.Itisimportanttounderstandtheheatavoidancemechanismsandtheirassociationswithleafmorpho-physiologicaltraitsthatallowcropstostaycoolandretainhighbiomassunderwarmanddryconditions.Weexamined20morpho-physiologicallydiversewheatgenotypesunderambientandelevatedtemperatures(Tair)toinvestigatewhetherincreasedwateruseleadstohighbiomassretentionduetoincreasedleafcooling.Anexperimentwasconductedunderwell-wateredconditionsintwopartiallycontrolledglasshouses.Wemeasuredplanttranspiration(Tr),leaftemperature(Tleaf),vaporpressuredeficit(VPD),and...