PesticideScienceandAdministration2023,44(7)农药研究QQQQQQQQQQQQQQuuuuuuuuuuuuuuEEEEEEEEEEEEEECCCCCCCCCCCCCChhhhhhhhhhhhhhEEEEEEEEEEEEEERRRRRRRRRRRRRRSSSSSSSSSSSSSS结结结结结结结结结结结结结结合合合合合合合合合合合合合合高高高高高高高高高高高高高高效效效效效效效效效效效效效效液液液液液液液液液液液液液液相相相相相相相相相相相相相相色色色色色色色色色色色色色色谱谱谱谱谱谱谱谱谱谱谱谱谱谱--------------串串串串串串串串串串串串串串联联联联联联联联联联联联联联质质质质质质质质质质质质质质谱谱谱谱谱谱谱谱谱谱谱谱谱谱法法法法法法法法法法法法法法测测测测测测测测测测测测测测定定定定定定定定定定定定定定牛牛牛牛牛牛牛牛牛牛牛牛牛牛肉肉肉肉肉肉肉肉肉肉肉肉肉肉中中中中中中中中中中中中中中甲甲甲甲甲甲甲甲甲甲甲甲甲甲拌拌拌拌拌拌拌拌拌拌拌拌拌拌磷磷磷磷磷磷磷磷磷磷磷磷磷磷及及及及及及及及及及及及及及其其其其其其其其其其其其其其代代代代代代代代代代代代代代谢谢谢谢谢谢谢谢谢谢谢谢谢谢物物物物物物物物物物物物物物残残残残残残残残残残残残残残留留留留留留留留留留留留留留梁景文,朱国柱,罗泽君,姜学涯,苑婷婷(深圳凯吉星农产品检测认证有限公司,广东深圳518000)收稿日期：2023-7-11作简介：梁景文，男，工程师，主要从事食品质量安全检测分析研究。联系电话：13823230534；E-mail：liangjw229@163.com。DeterminationofPhorateanditsMetabolitesResiduesinBeefUsingQuEChERSCombinedWithHPLC-MS/MSLiangJingwen,ZhuGuozhu,LuoZejun,JiangXueya,YuanTingting(ShenzhenCare-greenAgri-cultureProductsTesting&CertificationCo.,Ltd.,ShenzhenGuangdong518000,China)Abstract:Thisstudyaimstoestablishamethodforthedeterminationofphorate,phoratesulfoneandphoratesulfoxideinbeefusingQuEChERScombinedwithhighperformanceliquidchromatogra-phy-tandemmassspectrometry(HPLC-MS/MS).Thebeefsamplewasmixedwithacetonitrilecontaining1%aceticacid,frozenat-18℃for30min,andthensubjecttoQuEChERSextraction.Aftercentrifugation,thesupernatantunderwentfurtherpurificationwithaQuEChERSclean-upkitandvortexcentrifugationbeforebeinginjectedintoHPLC-MS/MSforanalysis.Underoptimizedconditions,phorateanditsmetabolitesexhibitedgoodlinearrelationshipswithintherangeof10~200...
