分析与测评2023年8月第4期Aug.2023,No.4香料香精化妆品FLAVOURFRAGRANCECOSMETICS112www.ffc-journal.com*基金项目：辽宁省检验检测认证中心青年人才创新创业项目（编号SC20210110）收稿日期：2023-01-12；修回日期：2023-02-28作者辽宁省药品检验检测院，辽宁沈阳110036…………………………王超李莹陆军矫筱蔓超高效液相色谱-串联质谱法测定化妆品中藤黄酸等5种成分*摘要采用超高效液相色谱-串联质谱（UPLC-MS/MS）技术，建立了化妆品中藤黄酸、转位藤黄酸、藤黄酸B、异藤黄酸及新藤黄酸的测定方法。样品经过体积分数75%甲醇水溶液超声提取后，以ZorbaxEclipsePlusC18为色谱柱、0.1%（体积分数）甲酸水-乙腈（体积比30∶70）为流动相，采用电喷雾离子（ESI）源、正离子扫描的多反应监测（MRM）模式进行定量分析。结果表明，藤黄酸等5种成分在5~250ng/mL范围内线性关系良好，相关系数（r）≥0.999，加标回收率为90.6%~117.9%，相对标准偏差（RSD）均小于10%。该方法简单、快速，为化妆品中藤黄酸等成分的检测提供了技术参考。关键词超高效液相色谱-串联质谱（UPLC-MS/MS）化妆品藤黄酸转位藤黄酸藤黄酸B异藤黄酸新藤黄酸DeterminationofFiveComponentsIncludingGambogicAcidinCosmeticsbyUltraPerformanceLiquidChromatographyTandemMassSpectrometry*WANGChaoLIYingLUJunJIAOXiaoman(LiaoningInstituteforDrugControl,Shenyang110036,Liaoning,China)Abstract:Anultraperformanceliquidchromatographytandemmassspectrometry(UPLC/MS-MS)methodforthedeterminationofgambogicacid,gambogellicacid,morellicacid,isogambogicacidandgambogenicacidincosmeticswasdeveloped.Afterultrasonicextractionwith75%methanol-water(involumefraction),thesamplewaselutedonZorbaxEclipsePlusC18columnwithmobilephaseconsistedof0.1%(involumefraction)formicacidsolution-acetonitrile(volumeration30:70).Forquantitation,theelectrosprayionization(ESI)sourcewasappliedtocarryoutthepositiveionscanningwithmultiplereactionmonitoring(MRM)mode.Theresultsshowedthatgoodlinearitieswereobtainedintheconcentrationrangeof5-250ng/mL,thecorrelationcoefficients(r)≥0.999.Therecoveriesatdifferentconcentrationswere90.6%-117.9%withtherelativestandarddeviations(RSDs)lessthan10%.Themethodissimpleandrapid,andprovidesatechnicalreferencefo...
