化学工程师Sum335No.8ChemicalEngineerDO1:10.16247/j.cnki.23-1171/tq.20230805科研与开发5-ALA光动力-热敏醇质体的制备工艺研究2023年第8期罗国平1,任婉婷1,闫梦茹1,孟会宁2（1.西安医学院药学院，陕西西安710021;2.山东步长医药销售有限公司，山东菏泽274418）摘要：优化5-氨基乙酰丙酸盐酸盐（5-ALA)醇质体的制备工艺。选用大豆卵磷脂及胆固醇作为囊材，采用乙醇注人法制备5-ALA醇质体。以热敏性、包封率、变形性、粒度分布等为评价指标，分别采用单因素实验和正交实验优化工艺条件。最优制备条件为：m(大豆卵碱脂):m(胆固醇)=9:1,m(囊材):m(药物)=23.3:1,无水乙醇加人体积为10mL，PBS溶液的pH值为7.4，搅拌时间为30min，超声时间为4min。本实验优化得到的5-ALA光动力一热敏醇质体包封率较高，变形性较好，粒度分布均匀。关键词：5-氨基乙酰丙酸盐酸盐；光动力作用；热敏醇质体；工艺优化；药剂学性质评价中图分类号：TQ469Studyonthepreparationprocessof5-ALAphotodynamic-thermosensitiveethosomes*(1.PharmaceuticalInstitute,Xi'anMedicalUniversity,Xi'an710021,Chinai2.ShandongBuchangMedicineCo.,Ltd.,Heze274418,China)Abstract:Tooptimizethepreparationconditionsof5-aminolevulinicacidhydrochloride(5-ALA)ethosomes.Soybeanlecithinandcholesterolwereusedascapsulematerialstoprepare5-ALAethosomesbyethanolinjectionmethod.Takingthermalsensitivity,encapsulationratio,deformabilityandparticlesizedistributionasevaluationindexes,theprocessconditionswereoptimizedbysinglefactortestandorthogonaltest.Theoptimumpreparationconditionswereasfollows:m(sobeaneathn)im(choea)=9:1,m(apiemeia)im(mainug)=23.3:1,thevolumeofanhydrousethanoladdedwas10mL,thepHofPBSsolutionwas7.4,thestirringtimewas30min,andtheultrasonictimewas4min.Theoptimized5-ALAphotodynamic-thermosensitiveethosomesobtainedinthisexperimenthadhighencapsulationefficiency,gooddeformabilityanduniformparticlesizedistribution.Keywords:5-ALA;photodynamiceffect;photodyamicaction;thermosensitiveethosomesiprocessoptimization;evaluationofpharmaceuticalproperties.光动力疗法(PDT)是运用特定波长的光来激发光敏药物，使其发生一定的光化学反应，导致机体细胞或分子发生形态及机能的改变，从而达到的诊断或治疗作用11。5-氨基乙...
