河南农业科学,2023,52(7)：136-143JournalofHenanAgriculturalSciencesdoi:10.15933/j.cnki.1004-3268.2023.07.0141株光合细菌的分离鉴定、培养基优化及脱氮特性研究靳鹏，和子涵，武婧玉，贾一然，张晓彤，张建新（河南师范大学水产学院，河南新乡453007)摘要：优化光合细菌发酵培养基，并研究其产酶特性和脱氮特性，以提升光合细菌在水质改良中的效果。采用双层平板法从河南省新乡市卫河水域底泥中分离筛选出1株光合细菌，命名为WH-1,通过菌株形态学观察及16SrDNA序列分析，确定分离菌株WH-1为沼泽红假单胞菌（Rhodopseudomonaspalustris)。为提高培养的WH-1菌液浓度,采用单因素和正交试验优化发酵培养基,得到最佳培养基配方：CH,CO0Na1.0g/L、NH4Cl0.5g/L、酵母浸粉3.0g/L、NaCI2.0g/L、K,HPO40.2g/L、NaHCO,3.0g/L、MgS040.2g/L。培养基优化之后，经流式细胞仪计数，WH-1菌液浓度达到4.37×10°个/mL，较优化前增长43.33%。产酶特性试验结果显示，菌株WH-1可以产纤维素酶、淀粉酶，但不能产蛋白酶。在高质量浓度的氨氮（NH3-N）、亚硝酸氮（NO,-N)环境中，WH-1菌株的NH,-N、NO,-N降解率分别达到49.89%和31.15%。综上，成功分离出1株性能优良的光合细菌WH-1，优化了培养基组成，该菌株展现出良好的产酶性能和脱氮能力。关键词：光合细菌；培养基优化；纤维素酶；淀粉酶；氨氮；亚硝酸氮中图分类号：S96IsolationandIdentificationofaPhotosyntheticBacteriumStrain,OptimizationofCultureMedium,andStudyofDenitrificationJINPeng,HEZihan,WUJingyu,JIAYiran,ZHANGXiaotong,ZHANGJianxin(CollegeofFisheries,HenanNormalUniversity,Xinxiang453007,China)Abstract:Inordertoenhancetheeffectsofphotosyntheticbacteriainwaterqualityimprovement,thefermentationmediumofphotosyntheticbacteriawasoptimizedandtheenzymeproductionanddenitrificationcharacteristicswerestudied.AstrainofphotosyntheticbacteriumnamedWH-1wasisolatedandscreenedfromthesedimentofWeiheRiverinXinxiangCity,HenanProvincebythedouble-layerplatemethod.Throughmorphologicalobservationand16SrDNAsequenceanalysis,theisolatedstrainWH-1wasidentifiedasRhodopseudomonaspalustris.ToincreasetheconcentrationofWH-1inliquidmedium,singlefactorandorthogonalexperimentswereutilizedtooptimizethefermentationmedium.Theoptimalmediumformulawasobtained:CH,C...
