花生学报2023,52（2）：52-60JournalofPeanutScience,Vol.52,No.2,2023DO1:10.14001/j.issn.1002-4093.2023.02.007BiocontrolPotentialityofEndophyticBurkholderiacepaciaagainstPeanutPodRotCausedbyPythiummyriotylumYUJing,SONGXin-ying,LIYing,HEKang,GUOZhi-qing,XUMan-lin,ZHANGXia",CHIYu-cheng(ShandongPeanutResearchInstitute,Qingdao266100,China)Abstract:PeanutpodrotcausedbyPythiummyriotylumresultsinsignificanteconomiclossesworldwideduringpeanutproduction.Biologicalcontrolisanalternativemeasuretocurrentchemicalcontrolmethods,whichareofteninefficientandhaveadverseenvironmentaleffects.Tocontrolthisdisease,39endophyticbacterialstrainsfromhealthypeanut(Arachishypogaea)rootswerescreenedfortheirantagonisticactivi-tiesagainstP.myriotylum.Fiveofwhichshowedefficientbiocontroleffects.Resultsshowed,theywereallidentifiedasBurkholderiacepaciaonthebasisof16SribosomalRNAandRecAgenesequences.A-mongthefiveB.cepaciastrains,PRI08manifestedthegreatestantagonisticactivityagainstP.myrioty-lummycelialgrowth.Ingreenhouseandfieldexperiments,infectedpeanutplantswithB.cepaciaPRI8inoculationreducedthediseaseincidenceby29.5%and11.83%,respectively.Moreover,B.cepaciaPRI08significantlypromotedpeanutgrowthbyenhancingthemainstemlength,lateralbranchlength,podnumber,plumppodrateandpodyieldby20.44%,19.98%,26.61%,20.36%and21.52%,respectively.ThisstudyrevealedthatB.cepaciaPRIo8hasthepotentialtobeusedasanagricultur-albiocontrolagentandplantgrowth-promotingbacterium.Keywords:Pythiummyriotylum;endophyte;Burkholderiabacteria;biologicalcontrol伯克氏菌对群结腐霉引起的花生果腐病的生防潜力于静，宋新颖，李莹，何康，郭志青，许曼琳，张（山东省花生研究所，山东青岛266100）摘要：花生果腐病是一种世界性的花生病害,群结腐霉是其主要致病菌之一,在花生生产过程中造成重大经济损失,生物防治是一种有效的绿色防控手段。为了控制花生果腐病的发生，从健康的花生植株上分离获得39株内生细菌，并筛选出5株对群结腐霉菌具有明显拮抗活性的菌株。结果表明,基于16S核糖体RNA序列和RecA序列，有5个菌株均被鉴定为洋葱伯克霍尔德菌（Burkholderiacepacia）,其中，菌株PRI08...
