疾病预防控制通报2023年2月第38卷第1期BullDisControlPrevFeb.,2023,Vol.38,No.1全自动碘分析仪测定尿碘方法的验证代涛娟，宋康四川省雅安市疾病预防控制中心，四川雅安625000摘要：目的通过使用全自动碘分析仪检测2022年尿碘标准物质，探讨全自动碘分析仪检测方法在大规模尿碘检测中的可行性。方法建立全自动碘分析仪的检测方法，对国家碘缺乏病参照实验室碘标准冻干尿样（GBW09108u、GBW09110z）的检测结果在标准曲线线性范围、相关系数、检出限、结果准确度、精密度方面进行验证。结果该方法在0～300μg/L范围内线性关系良好，相关系数r=0.9998，检出限为2.73μg/L，对尿碘低值和高值2种浓度的标准物质的测定结果准确度符合要求，精密度分别为2.7%和1.7%。结论采用全自动尿碘分析仪检测尿碘，方法简单，自动化程度高、污染较小，适用于大批量样本尿碘检测。关键词：尿碘；检测；分析仪中图分类号：R446.12文献标识码：A文章编号：1000⁃3711（2023）01⁃0083⁃02ValidationofdetectionmethodwithautomaticiodineanalyzerfordeterminationofurineiodineDAITao-juan，SONGKangYaanMunicipalCenterforDiseaseControlandPrevention,Yaan,Sichuan625000,ChinaCorrespondingauthor:SONGKang,E-mail:517968562@qq.comAbstract:ObjectiveTodetectthenationalstandardurinaryiodinereferencematerialsbyautomaticiodineanalyzerin2022,anddiscussthefeasibilityoftheautomaticiodineanalyzerusinginlarge-scaleurineiodinedetection.MethodsTheiodinedetectionmethodwithautomaticiodineanalyzerwasestablished,andthedetectionresultsofthefreeze-driedurinesamples(GBW09108u,GBW09110z)fromthenationaliodinedeficiencydisordersreferencelaboratorywereverifiedinstandardcurvelinearrange,correlationcoefficient,detectionlimit,accuracyandprecision.ResultsThelinearrelationwasfineintherangeof0-300μg/L,withthecorrelationcoefficientr=0.9998.Thedetectionlimitwas2.73μg/L.Thedetectionresultsofthestandardmaterialswithlowandhighconcentrationofurinaryiodinereachtheaccuracyrequirement,andtheprecisionwas2.7%and1.7%,respectively.ConclusionsThemethodofurinaryiodinedetectionwithautomaticurineiodineanalyzerissimple,withhighdegreeofautomationandlesspollution,andaresuitableforurineiodinedetectioninlargenumberofurines...
