Isolation，identificationanddegradationcharacteristicsoforganicmatterdegradingbacteriainblackandodorouswaterMAXing-guan，DONGChang，TANGYu-lan，HEYa-ting，WANGXiao-hua(SchoolofMunicipalandEnvironmentalEngineering，ShenyangJianzhuUniversity，Shenyang110168，China)Abstract:Thisworkaimedtoexcavatehigh-efficiencyorganicmatterdegradationstrainfromblackandodorouswater，whichwasappliedtolow-concentrationorganicpollutedblackandodorouswater，andevaluateitsabilityandcharacteristicstodegradeorganicmatterinblackandodorouswater．Blackandodorouswaterorganicmatterdegradingstrainwasobtainedbyroutineisolationandscreeningofbacteria，andthestrainwasidentifiedthroughmorphologicalobservationand16SrDNAgenesequence．Thesingle-testexperimentswereusedtooptimizecultivationconditions．Furthermore，undertheoptimizedcultivationconditions，thegrowthcurveofthestrainwasmeasuredtodeterminetheoptimalinoculationpoint．ThedegradationabilityofthestrainonorganicmatterinblackandodorouswaterandthedegradationcharacteristicsunderdifferentinitialbacterialconcentrationsandcarbonsourceswereevaluatedbymeasuringCODCrremovalrateandcolonynumber．Ahighdegradationrateoforganicmatterinblackandodorouswaterbacteriumawasisolatedandscreenedfromthesedimentofblackandodoroussection(RiverRenjing，Shenyang)，whichwasidentifiedasBacillustoyonensis．TheoptimalgrowthconditionsafteroptimizationwerepH7.0，equipmenttheliquidvolumewas25%，thetemperaturewas32℃．Underthiscondition，theoptimalinoculationpointwasculturedfor12h．Theorganicmatterdegradationcharacteristicsofstrainashowedthattheremovalrateoforganicmatterinblackandodorouswaterreached64.75%whenthereactionwascarriedoutinashakerat30℃and120r/minfor4days．Underthesameexperimentalconditions，increasingtheinitialconcentrationofbacteriaresultedinincreasinginCODCrremovalrateandthendecreasing．Whentheinitialbacterialconcentrationwas1%(volumefraction)，CODCrremovalratewashigher．Strainahaddifferentutilizationofdifferentcarbonsourcesandhadhigherutilizationofsmallmolecularweight．Th...
