19工艺技术《食品工业》2023年第44卷第2期响应面优化山楂叶总黄酮的提取工艺及体外降脂研究牛成伟*，宋涵瑶，顾浩，李长春，雷小婷江苏食品药品职业技术学院药学院（淮安223001）摘要以山楂叶为研究对象,研究其总黄酮提取工艺及降脂活性。在单因素试验基础上,采用Box-Behnken响应面法对山楂叶总黄酮的提取工艺进行优化,考察乙醇体积分数、液料比和提取时间对其总黄酮产率的影响,以胰脂肪酶抑制率为指标评价山楂叶总黄酮的降脂活性。结果表明,最佳提取条件为乙醇体积分数56%、液料比28︰1(mL/g)、提取时间34min,此时山楂叶总黄酮产率为4.43%。活性测定结果表明,山楂叶总黄酮具有较好的降脂活性,且具有一定的浓度依赖性。优选的山楂叶总黄酮提取工艺方法可行,其总黄酮具有一定降脂活性。关键词山楂叶;响应面;总黄酮;提取;降脂OptimizationfortheExtractionProcessoftheFlavonoidsfromtheHawthornLeavesbyResponseSurfaceMethodologyandEvaluationofHypolipidemicActivityNIUChengwei*,SONGHanyao,GUHao,LIChangchun,LEIXiaotingPharmacyCollegeofJiangsuFoodandPharmaceuticalScienceCollege(Huaian223001)AbstractOptimizetheextractionprocessandevaluatehypolipidemiceffectsoftotalflavonoidsinthehawthornleaves.Basedonasinglefactortest,theethanolconcentration,theratioofsolventtomaterialandtheextractiontimewereusedasthefactors,andtheBox-Behnkenwasusedtodesigntheextractionprocess.Theinhibitionrateofpancreaticlipasewasusedtoassessthehypolipidemiceffectoftotalflavonoidsinthehawthornleaves.Theresultshowedthattheoptimalextractionconditionwasasfollows:ethanolconcentration,theratioofsolventtomaterialandextractiontimewere56%,28︰1(mL/g),and34min,respectively.Undertheabovecondition,theexperimentalyieldoftotalflavonoidsinthehawthornleaveswas4.43%.Furthermore,totalflavonoidsinthehawthornleavesexhibitedsignificanthypolipidemiceffectsinvitroinadose-dependentmanner.Theoptimumextractionprocessoftheflavonoidsfromthehawthornleavesisfeasible,andtotalflavonoidsinthehawthornleaveshavesignificanthypolipidemiceffects.Keywordshawthornleaves;responsesurfacemethodology;totalflavonoids;extraction;hypolipidemiceffect*通信作者；基金项目：江苏省大学生创新创业训练计划（202113104038Y...
