小尾寒羊与双乾肉羊的瘤胃菌群差异分析彭洪洋1，李玉梅1*，赵中利2，张英1，白春艳1，闫守庆1，马惠海2*（1.吉林大学动物科学学院,长春130062；2.吉林省农业科学院畜牧兽医研究所,吉林公主岭136100）摘要：本试验旨在研究小尾寒羊(STH)及双乾肉羊(SQ)瘤胃微生物区系特征及品种间差异。随机挑选出生日期相近、初体重相近、健康的小尾寒羊和双乾肉羊各6只，采用16SrDNA扩增子测序方法，探究两个绵羊品种之间瘤胃微生物的差异，预测其微生物功能。结果表明，SQ组瘤胃菌群多样性和丰富性均高于STH组；主坐标分析和非加权组平均法聚类分析显示组间具有差异性。两个品种绵羊瘤胃菌群在门水平上组成相似，优势菌门均为拟杆菌门、厚壁菌门及变形菌门。对差异菌群进行KEGG功能预测，结果显示，双乾肉羊瘤胃微生物功能大部分高于小尾寒羊。从上述分析来看，双乾肉羊瘤胃菌群种类及丰度均高于小尾寒羊，这可能导致了双乾肉羊饲料利用率更高。关键词：小尾寒羊；瘤胃微生物；16SrDNA；菌群结构中图分类号：S826文献标识码：A文章编号：2096-5877（2023）01-0050-04AnalysisontheDifferenceofRuminalMicroflorabetweenSmall-TailHanSheepandShuangqianMuttonSheepPENGHongyang1,LIYumei1*,ZHAOZhongli2,ZHANGYing1,BAIChunyan1,YANShouqing1,MAHuihai2*（1.CollegeofAnimalScience,JilinUniversity,Changchun130062;2.InstituteofAnimalHusbandryandVeterinary,JilinAcademyofAgriculturalSciences,Gongzhuling136100,China)Abstract：Thepurposeofthisexperimentistoinvestigatetheruminalmicrofloracharacteristicsanddifferencesbe⁃tweenSmall-tailHansheep(STH)andShuangqianmuttonsheep(SQ).6STHand6SQwererandomlyselected,whichweresimilarbirthdate,initialbodyweightandgoodhealth.The16SrDNAampliconsequencingmethodwasappliedtoexplorethemicrobialdifferencesbetweentherumenoftwobreedsandpredictthemicrobialfunction.TheresultsindicatedthatthebacterialdiversityandrichnessofrumenofShuangqianmuttonsheeparehigherthanthoseofSmall-tailHansheep.Theprincipalcoordinateanalysis(PCoA)andUPGMAclusteranalysisshowedthatthebacteriacommunityintwogroupswasdifferent.ThecompositionofrumenmicrofloraofthetwobreedsofsheepissimilaratthephylumlevelanddominantphylawereBacteroidetes,FirmicutesandProteobacteria.TheresultsofKEGGpathw...
