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哮喘患儿外周血T淋巴细胞亚...其与ACT评分的相关性研究_罗婷婷.pdf
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哮喘 患儿外周血 淋巴细胞 ACT 评分 相关性 研究 婷婷
Hainan Med J,Feb.2023.Vol.34,No.4海南医学2023年2月第34卷第4期8Chen FY,Gao YT,Chen YH.Analysis of clinical characteristics andprognostic factors of status epilepticus convulsions J.Hainan Medi-cal Journal,2020,31(5):597-599.陈飞燕,高玉婷,陈友海.惊厥性癫痫持续状态的临床特征及预后影响因素分析J.海南医学,2020,31(5):597-599.9Katyayan A,Diaz-Medina G.Epilepsy:Epileptic Syndromes andTreatment J.Neurol Clin,2021,39(3):779-795.10 Magliozzi R,Marastoni D,Calabrese M.The BAFF/APRIL systemas therapeutic target in multiple sclerosis J.Expert Opin Ther Tar-gets,2020,24(11):1135-1145.11 Chen QY,Zhang JQ,Gong Q.The regulation of HMGB1 on lympho-cyte function J.Immunological Jouranl,2020,36(4):364-368.陈秋悦,张建强,龚权.HMGB1对淋巴细胞功能的调控J.免疫学杂志,2020,36(4):364-368.12 Han YW,Wang CC,Li XM.Oleanolic acid modulates HMGB1/TLR4/NF-B mediated inflammatory pathway to reduce early braininjury after subarachnoid hemorrhage J.Practical Pharmacy andClinical Remedies 2020,23(1):5-9.韩雨薇,王晨辰,李晓明.齐墩果酸调节HMGB1/TLR4/NF-B介导的炎症通路减轻蛛网膜下腔出血后的早期脑损伤J.实用药物与临床,2020,23(1):5-9.13 Zhang XY,Wang XJ,Fu XD,et al.Serum levels of HMGB1,TLR2and TLR4 in patients with mild cognitive impairment and their clini-cal significance J.Chin Gen Prac,2019,22(6):673-677.张旭阳,王新军,付旭东,等.轻型颅脑损伤伴轻度认知功能障碍患者血清HMGB1和TLR2及TLR4水平及其临床意义研究J.中国全科医学,2019,22(6):673-677.14 Jing Y,Ai ShanJiangYSFJ,LU MJ.Expression of serum BAFF inepileptic patients and its correlation with IFN-and IL-10 levels J.Hebei Medical Journal,2020,42(1):88-91.景燕,艾山江玉苏甫江,陆明佳.癫痫患者血清BAFF表达及与IFN-、IL-10水平的相关性J.河北医药,2020,42(1):88-91.15 Ye JY,Yang LH.Expression of serum high mobility group protein 1and Toll-like receptor 4 in patients with epilepsy and its influencingfactors J.Journal of Sun Yat-sen University(Medical Sciences),2021,42(6):839-845.叶嘉颖,杨炼红.癫痫患者血清高迁移率族蛋白1与TOLL样受体4的表达及影响因素J.中山大学学报(医学科学版),2021,42(6):839-845.(收稿日期:2022-05-07)哮喘患儿外周血T淋巴细胞亚群、IL-5、IL-25水平变化及其与ACT评分的相关性研究罗婷婷,胡梦娇,赵静利,程雨嘉,李青宝鸡市人民医院儿科,陕西宝鸡721000【摘要】目的观察哮喘患儿外周血T淋巴细胞亚群、白介素-5(IL-5)、白介素-25(IL-25)水平,并分析其与哮喘控制测试评分表(ACT)评分的关系。方法选取2017年5月至2020年6月宝鸡市人民医院收治的115例哮喘患儿作为观察组,依据患儿的临床表现分为缓解期组47例与急性发作期组68例,另选取同期在我院体检的50例健康儿童为对照组,检测两组儿童外周血T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)、IL-5及IL-25水平,采取ACT问卷调查患儿哮喘控制情况,并使用Pearson相关性检验法分析患儿T淋巴细胞亚群、IL-5、IL-25水平与ACT评分相关性。结果急性发作期组患儿的CD8+为(37.025.24)%,明显低于缓解期组的(40.196.23)%和对照组的(43.055.03)%,缓解期组又明显低于对照组,差异均有统计学意义(P0.05);急性发作期组患儿的CD4+、CD4+/CD8+分别为(54.787.06)%、1.570.28,明显高于缓解期组(51.167.75)%、1.400.25和对照组(47.615.02)%、1.180.17,缓解期组又明显高于对照组,差异均有统计学意义(P0.05);急性发作期组患儿的IL-5、IL-25分别为(74.9212.13)pg/mL、(57.248.96)pg/mL,明显高于缓解期组(67.3810.54)pg/mL、(48.738.05)pg/mL和对照组(50.869.85)pg/mL、(44.687.12)pg/mL,缓解期组又明显高于对照组,差异均有统计学意义(P0.05);急性发作期组患儿的ACT评分为(16.152.76)分,明显低于缓解期组的(20.842.37)分,差异有统计学意义(P0.05);经Pearson相关性分析结果显示,CD8+与ACT评分呈显著正相关(P0.05),CD4+、CD4+/CD8+、IL-5、IL-25与ACT评分呈显著负相关(P0.05),具有可比性。哮喘患儿依据临床表现分为缓解期组47例和急性发作期组68例。本研究经我院医学伦理委员会批注。1.2方法(1)检测方法:抽取对照组儿童入院检查时、观察组入院后空腹静脉血5 mL,采取3 000 r/min速率予以离心处理,取血清。通过八色流式细胞仪(型号:MACSQuant,购自德国Miltenyi Biotec)完成T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)的检测。通过Thermo Multiskan MK3酶标仪,使用酶联免疫吸附试验双抗体夹心法进行IL-5与IL-25水平检测,相关试剂盒来自美国R&D公司,严格依据说明书完成操作过程。(2)采取哮喘控制测试评分表(ACT)7评估患儿哮喘控制情况,总分25分,控制良好:25分;基本控制:2024分;未得到控制:20分。1.3观察指标比较缓解期组、急性发作期组与对照组儿童外周血T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)、IL-5、IL-25水平;比较缓解期组、急性发作期组儿童ACT评分。1.4统计学方法应用 SPSS22.0统计软件分析数据。计数资料组间比较采用2检验;计量资料符合正态分布,以均数标准差(x-s)表示,组间两两比较采用独立样本t检验,多组比较采用单因素方差分析法,多组两两比较采用LSD-t检验法;采取Pearson相MethodsA total of 115 asthma children admitted to Baoji Peoples Hospital from May 2017 to June 2020 were selectedas the observation group,which were divided into the remission stage group(n=47)and the acute attack group(n=68)ac-cording to clinical manifestations.In addition,50 healthy children who were examined during the same period were select-ed as the control group.The levels of peripheral blood T lymphocyte subsets(CD3+,CD4+,CD8+and CD4+/CD8+),IL-5,and IL-25 in the two groups were detected.The situation of asthma control in children was investigated by ACT.The cor-relation of T lymphocyte subsets,IL-5,and IL-25 levels with ACT score was analyzed by Pearson method.ResultsThelevel of CD8+in the acute attack group was(37.025.24)%,which was significantly lower than(40.196.23)%in the re-mission stage group and(43.055.03)%in the control group,and the levels in the remission stage group was significant-ly lower than that in the control group(P0.05).The levels of CD4+and CD4+/CD8+in the acute attack group were(54.787.06)%and 1.570.28,which was significnatly higher than(51.167.75)%,1.400.25 in the remission stage groupand(47.615.02)%,1.180.17 in the control group,and the levels in the remission stage group were significantly higherthan in the control group(P0.05).The levels of IL-5 and IL-25 in the acute attack group were(74.9212.13)pg/mL and(57.248.96)pg/mL,which were significantly higher than(67.3810.54)pg/mL,(48.738.05)pg/mL in the remissionstage group and(50.869.85)pg/mL,(44.687.12)pg/mL in the control group,and the levels in the remission groupwere significantly higher than those in the control group(P0.05).The ACT score in the acute attack group was(16.152.76)points

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