血清TRAF6、Talin...孕妇不良妊娠结局的预测价值_朱小红.pdf

东南大学学报(医学版)J Southeast Univ(Med Sci Edi)2023，Feb;42(1):85-91 J 现代医学，2022，50(2):163-168 9KOFANOVA O，HENY E，AGUILA QUESADA，et alIL8 and IL16 levels indicate serum and plasma qualityJ Clin Chem Lab Med，2018，56(7):1054-1062 10李自强，何引章，陶勇 细胞因子检测方法研究进展及其在眼内液检测中的应用J 中华眼科医学杂志(电子版)，2018，8(3):140-144 11KANY S，VOLLATH J T，ELJA B Cytokines in inflamma-tory disease J Int J Mol Sci，2019，20(23):6008 12ISNAD S，HATTON E X，IANNETTA M，et al Cell-associ-ated HIV cross-presentation by plasmacytoid dendritic cells ispotentiated by noncognate CD8+T cell preactivationJ JImmunol，2021，207(1):15-22 13 VEMA A K，BAUE C，PALANI S，et al IFN-drivesTNF-hyperproduction and lethal lung inflammation duringantibiotic treatment of postinfluenza Staphylococcus aureuspneumonia J J Immunol，2021，207(5):1371-1376 14BODON J，ALIBETI S，FENANDEZ-BOTAN，et alUnderstanding the roles of cytokines and neutrophil activityand neutrophil apoptosis in the protective versus deleteriousinflammatory response in pneumoniaJ Int J Infect Dis，2013，17(2):e76-83 15JIN J O，HAN X，YU Q Interleukin-6 induces the generationof IL-10-producing Tr1 cells and suppresses autoimmune tis-sue inflammation J J Autoimmun，2013，40:28-44 16CHEN Y Q，LI P C，PAN N，et al Tumor-released autophago-somes induces CD4+T cell-mediated immunosuppression viaa TL2-IL-6 cascadeJ J Immunother Cancer，2019，7(1):178 17OSSE E C，OLEINIKA K，TONON S，et al egulatory Bcells are induced by gut microbiota-driven interleukin-1 betaand interleukin-6 productionJ Nat Med，2014，20(11):1334-1339 收稿日期2022-09-21修回日期2022-11-14 基金项目河南省高等学校重点科研项目计划(22A320049)作者简介朱小红(1986 )，女，宁夏回族自治区人，主治医师。E-mail:zhuxiaohong1986z163 com 引文格式朱小红，刘惠娜，赵珂，等 血清 TAF6、Talin1 水平对子痫前期孕妇不良妊娠结局的预测价值 J 东南大学学报(医学版)，2023，42(1):85-91 论著 血清 TAF6、Talin1 水平对子痫前期孕妇不良妊娠结局的预测价值朱小红，刘惠娜，赵珂，李胜男(郑州大学第一附属医院 产科，河南 郑州450000)摘要目的:探究子痫前期孕妇血清中肿瘤坏死因子受体相关因子 6(TAF6)、踝蛋白-1(Talin1)的表达水平，以及评估两者对不良妊娠结局的预测价值。方法:收集2020 年1 月至2022 年1 月在本院接受治疗的94例子痫前期孕妇作为子痫前期组，另选取同时间健康的 70 例孕妇作为对照组。将子痫前期组根据妊娠结局分为不良组和正常组。通过实时荧光定量 PC(qT-PC)检测各组血清 TAF6、Talin1 的相对表达水平;并对子痫前期孕妇不良妊娠结局的影响因素进行单因素和多因素分析;绘制受试者工作特征(OC)曲线分析血清 TAF6、Talin1 水平对子痫前期孕妇不良妊娠结局的预测价值。结果:与对照组相比，子痫前期组TAF6 水平和 Talin1 相对表达量显著升高(P 0 05);且子痫前期组妊娠结局总不良率显著高于对照组(P 0.05)。单因素分析结果表明，血压、24 h 尿蛋白定量、TAF6 水平和 Talin1 水平均是影响子痫前期孕妇不良妊娠结局的因素(P 0 05);多因素 Logistic 回归分析结果显示，血清 TAF6、Talin1 高表达是影响子痫前期孕妇不良妊娠结局的危险因素;OC 曲线结果显示，血清 TAF6 和 Talin1 预测子痫前期孕妇不良妊娠结58局曲线下面积(AUC)分别为 0 835、0 872，当两者联合时其 AUC 为 0 931，高于两者单独预测(P 0 05)。结论:子痫前期孕妇血清 TAF6、Talin1 水平显著升高，与子痫前期孕妇不良妊娠结局密切相关，两者联合检测对预测子痫前期孕妇不良妊娠结局具有较好的效能。关键词子痫前期;肿瘤坏死因子受体相关因子 6;踝蛋白-1;不良妊娠结局;预测价值 中图分类号714 44 文献标志码A 文章编号1671-6264(2023)01-0085-07doi:10 3969/j issn 1671-6264 2023 01 012Predictive value of serum TAF6 and Talin1 levels on adverse pregnancyoutcomes in pregnant women with preeclampsiaZHU Xiaohong，LIU Huina，ZHAO Ke，LI Shengnan(Department of Obstetrics，the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450000，China)AbstractObjective:To investigate the expression levels of tumor necrosis factor receptor-associated factor 6(TAF6)and Talin1 in the serum of pregnant women with preeclampsia，and to evaluate their predictive value foradverse pregnancy outcomes Methods:From January 2020 to January 2022，94 pregnant women with preeclamp-sia who received treatment in our hospital were allocated into preeclampsia group，70 healthy pregnant women con-trol group The preeclampsia group was divided into normal group and abnormal group in terms of the pregnancyoutcome The relative expression levels of serum TAF6 and Talin1 in each group were detected by real-time fluo-rescence quantitative PC(qT-PC)method;univariate and multivariate analyses were performed to analyze theinfluencing factors of adverse pregnancy outcomes in pregnant women with preeclampsia;receiver operating charac-teristic(OC)curve was drawn to observe the predictive value of serum TAF6 and Talin1 levels for adverse preg-nancy outcomes in pregnant women with preeclampsia esults:Compared with the control group，the relative ex-pressions of TAF6 and Talin1 in the preeclampsia group were obviously increased(P 0 05);and the total ad-verse pregnancy outcome rate in the preeclampsia group was obviously higher than that in the control group(P 0.05);the results of univariate analysis showed that blood pressure，24-hour urine protein quantification，TAF6and Talin1 levels were all the factors affecting the adverse pregnancy outcomes of pregnant women with preeclampsia(P 0 05);multivariate Logistic regression analysis showed that the high expression of serum TAF6 and Talin1was a risk factor for adverse pregnancy outcomes in pregnant women with preeclampsia;OC curve results showedthat the area under the curve(AUC)of serum TAF6 and Talin1 for predicting adverse pregnancy outcomes in pre-eclampsia pregnant women was 0 835 and 0 872，respectively，and when the two were combined，the AUC was0.931，which was higher than that of the two alone(P 0 05)Conclusion:The expression levels of TAF6 andTalin1 in the serum of pregnant women with preeclampsia are obviously increased，which are cl